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EMAGE:4962

Wnt5a wingless-related MMTV integration site 5A ( MGI:98958)
TS19 (11.5 dpc)
in situ hybridisation

Data Images
EMAGE:4962 EMAGE:4962 EMAGE:4962 EMAGE:4962 EMAGE:4962
Raw signal output, as movie. All sections along the X-axis, as movie. All sections along the Y-axis, as movie. All sections along the Z-axis, as movie. Photograph prior to 3D imaging.
EMAGE:4962 EMAGE:4962 EMAGE:4962
Photograph prior to 3D imaging. Photograph prior to 3D imaging. Sample morphology following 3D imaging, as movie

View 3D opt image: EMAGE genex expression entry
Download 3D images in woolz format
Expression pattern clarity: two stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
This is a traditionally produced wholemount in situ hybridisation stained using alkaline phosphatase + NBT/BCIP. It has been imaged using Optical Projection Tomography. The visible channel depicting the signal has been false-coloured green and the fluorescence channel depicting autofluorescence has been false-coloured red.
Expression Pattern Description
Spatial Annotation:
EMAGE:4962Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
4962_wholemount_moderate_3D_1.wlz
4962_wholemount_weak_3D_1.wlz
4962_wholemount_notDetected_3D_1.wlz
4962_wholemount_strong_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:4962_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
embryo
detected detected
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:Wnt5a_probeC
Entity Detected:Wnt5a, wingless-related MMTV integration site 5A ( MGI:98958)
Sequence:sense strand is shown

>Wnt5a_probeC
GTGGTTTAATGGTGCCTGATATCTCAGTTTTTTTGTATATAACATATATATAAATATACATATATAAATA
TAGATATAATTATATCTCAGTGCAGTCTGGGATTTAGACCTACAGTTTTCTCTGGGCTTGCTCTCTGCCT
GGAGTATCGTCCTTCATTGCAGTCCAATTGGGATTTCTTTTTTTCCAAAAATTTTGAGTCTTAACATTGA
CCTGTGACAGGATCCTACCACGAATACCAGGAAGCAAGCTAAGACTCGGAGGAAGCTCTCAGGGCTCATG
TCCTGAATGTATGTTGGTTAGAAAGTAGCCTTTCTGCTTCCTGCCCATGGCCAGTTCTCCACCCTCTCTT
TGGTGTTCTTTGTGGGGAGGGCACTGTGGTTTGTCGCAGCCCTGGACTTCGAGAGGCTCCCAGAACCCAG
GATCACCAGCCTCCTGTCTGTTTGCTTCACTCCTTTCCCAGGGAGGACTTGGGACTGTCCTGTCTGACAG
GACGGATCTGAGTTCCCGAAGCAAAC
nt 29337556 - nt 29338071 of NC_000080.5
Notes:These primers were used to generate the in vitro transcription template by PCR of mouse whole genomic DNA. They contain T7 or T3 promotor sequences and amplify a region corresponding to a portion of the 3'UTR. The antisense probe was transcribed using the T7 polymerase. T3 forward primer (sense) 5'-3' AATTAACCCTCACTAAAGGTGTGGTTTAATGGTGCCTGA T7 reverse primer (antisense) 5'-3' TAATACGACTCACTATAGGTTTGCTTCGGGAACTCAGAT product size: 516bp
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:CD-1
Age:11.5 dpc
Theiler Stage:TS19
Mutations:none (wild-type)
Preparation:opt
Procedures
Fixation:4% paraformaldehyde
Embedding:agarose
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Jacqueline Ramsay, David R Fitzpatrick
Principal investigator:David R FitzPatrick, Medical Research Council, Human Genetics Unit MRC Western General Hospital Crewe Road, Edinburgh, Scotland EH4 2XU
Submitted by:Jacqueline Ramsay, Medical Research Council, Human Genetics Unit MRC Western General Hospital Crewe Road, Edinburgh, Scotland EH4 2XU
Experiment type:screen
Links: Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceFACEBASE