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EMAGE:4967

Meis2 Meis homeobox 2 ( MGI:108564)
TS20 (12.5 dpc)
in situ hybridisation

Data Images
EMAGE:4967 EMAGE:4967 EMAGE:4967 EMAGE:4967 EMAGE:4967
Raw signal output, as movie. All sections along the X-axis, as movie. All sections along the Y-axis, as movie. All sections along the Z-axis, as movie. Photograph prior to 3D imaging.
EMAGE:4967 EMAGE:4967 EMAGE:4967
Photograph prior to 3D imaging. Photograph prior to 3D imaging. Sample morphology following 3D imaging, as movie

View 3D opt image: EMAGE genex expression entry
Download 3D images in woolz format
Expression pattern clarity: two stars
Notes:
This is a traditionally produced wholemount in situ hybridisation stained using alkaline phosphatase + NBT/BCIP. It has been imaged using Optical Projection Tomography. The visible channel depicting the signal has been false-coloured green and the fluorescence channel depicting autofluorescence has been false-coloured red.
Expression Pattern Description
Text Annotation:
StructureLevelPatternNotes
embryo
detected detected
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MEIS2_T7
Entity Detected:Meis2, Meis homeobox 2 ( MGI:108564)
Sequence:sense strand is shown

>MEIS2_T7
GTGAGGAACACGTGCTGAGAAAAGAGGACCCCATGGACATACAATACCAATTCCACAGCAGATCTGATAC
TAGCAAAAACATTCTTTTTTTTTTTTTCAATTGAGGTAAACACATAGAATATCTAACATGAAACAATTAA
AAGACCGAACTCTGTACGAAGTTTGTTACAGTATTCTCTTGCTCCTTTTTATCCCCCAAGCTTTGAGTTC
CTGATAGAATCCTACTTATGGTGCAACGACCATGAATAACTTGTGTGTGTGTGTGTGTGTATGTGCGTTG
AGGAAAGCTGCCCAACTTAAGATTGTTTTGTCCACAGCCAAGGCTCAGAACTTGAAGAAGCCTTCGCTCT
GTCTCTAGATCTTCACTGGCAAGCTCTTTGAAGAACAGTCCCCCAAAATCATAATAAGAATTGGCTTATG
AAGCACAAACTATAAAGATCTGTTTGAAACTAAAGGACACATGGACGGGGGGAACAAAGGCCGGGCATGA
AAACACATTCTAGCCGCTGGAGT
nt 115554644 - nt 115555156 of NC_000068.5
Notes:This probe was transcribed from template produced by a PCR of whole genomic DNA using primers incorporating T3 and T7 promotors. The antisense probe was transcribed using the T7 polymerase. T3- 5' AATTAACCCTCACTAAAGGACTCCAGCGGCTAGAATGTG 3' T7- 5' TAATACGACTCACTATAGGGTGAGGAACACGTGCTGAGA 3' Product size: 513bp
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:CD-1
Age:12.5 dpc
Theiler Stage:TS20
Mutations:none (wild-type)
Preparation:opt
Procedures
Fixation:4% paraformaldehyde
Embedding:agarose
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Jacqueline Ramsay, David R FitzPatrick
Principal investigator:David R FitzPatrick, Medical Research Council, Human Genetics Unit MRC Western General Hospital Crewe Road, Edinburgh, Scotland EH4 2XU
Submitted by:Jacqueline Ramsay, Medical Research Council, Human Genetics Unit MRC Western General Hospital Crewe Road, Edinburgh, Scotland EH4 2XU
Experiment type:screen
Links: Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceFACEBASE