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EMAGE:4973

Dach1 dachshund 1 (Drosophila) ( MGI:1277991)
TS17 (10.5 dpc)
in situ hybridisation

Data Images
EMAGE:4973
Fig5a(10.5) Copyright: Reprinted with permission from Elsevier from [doi:10.1016/S0925-4773(98)00071-9] Mech Dev 74(1-2): 121-31, Hammond KL; Hanson IM; Brown AG; Lettice LA; Hill RE, Mammalian and Drosophila dachshund genes are related to the Ski proto-oncogene and are expressed in eye and limb. Copyright 1998. [PMID:9651501]

Expression pattern clarity: two stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
Image annotations: arrow indicates rib primordia.
Expression Pattern Description
Spatial Annotation:
EMAGE:4973Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
4973_wholemount_moderate_3D_1.wlz
4973_wholemount_weak_3D_1.wlz
4973_wholemount_notDetected_3D_1.wlz
4973_wholemount_strong_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:4973_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
neural tube
detected detected
Dach is expressed throughout the length of the neural tube. There is also expression of Dach within the dorsal root ganglia extending to the region where the ganglia join the neural tube. The expression domain of Dach is mainly within the mantle of the neural tube, with expression in dorsal, lateral and ventral regions. There is also a dorsal region of expression within the ventricular zone.
trigeminal v ganglion
detected detected
glossopharyngeal ix ganglion
detected detected
vagus x ganglion
detected detected
thoracic vertebral pre-cartilage condensation
detected detected
regionalRib primordia expression is seen at E10.5.
forelimb bud
detected detected
regionalexpression can be seen in both the posterior and anterior of the limb bud, with the anterior region extending centrally into the core.
hindlimb bud
detected detected
regionalexpression can be seen in both the posterior and anterior of the limb bud, with the anterior region extending centrally into the core.
head mesenchyme derived from neural crest
detected detected
regionalDach is expressed in the mesenchyme surrounding the eye which is predominantly neural-crest derived.
lens pit
not detected not detected
homogeneous
optic cup
detected detected
regionalDach expression also occurs within the neural retina but not in the lens or the retinal pigmented epithelium (RPE).
telencephalon
detected detected
Dach is expressed in the telencephalon including the olfactory bulbs.
diencephalon
not detected not detected
homogeneous
nose
detected detected
regionalDach is expressed in the telencephalon including the olfactory bulbs.
dorsal root ganglion
detected detected
There is also expression of Dach within the dorsal root ganglia extending to the region where the ganglia join the neural tube.
embryo
detected detected
regionalDach is expressed in a punctate pattern on the ventral side of the embryo between the fore and hind limbs, in the rib primordia.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:Dach1 probeB
Entity Detected:Dach1, dachshund 1 (Drosophila) ( MGI:1277991)
Sequence:sense strand is shown

>Dach1 probeB
ACCCTCTCATTCCTGTCAGCCTACCTCCAGCATCTGTCACCATGGCAATGAGTCAGATGAACCACCTTAG
CACCATTGCAAATATGGCGGCGGCAGCACAAGTTCAGAGTCCTCCATCCAGGGTGGAGACATCTGTTATT
AAGGAGCGTGTTCCCGACAGCCCCTCGCCTGCTCCATCTCTGGAGGAGGGCCGGAGGCCCGGCAGCCACC
CATCCTCACACCGCAGCAGCAGTGTGTCCAGCTCCCCGGCGCGGACTGAGAGTTCTTCCGACAGAATCCC
TGTCCATCAGAATGGCCTGTCCATGAACCAGATGCTTATGGGTTTATCCCCAAATGTGCTTCCTGGGCCA
AAGGAGGGGGATTTGGCTGGTCATGACATGGGGCATGAGTCAAAACGGATCCACATTGAAAAAGATGAGA
CCCCACTTTCCACACCAACCGCAAGAGACAGCATCGACAAACTTTCTCTAACTGGGCATGGACAACCGCT
ACCTCCCGGCTTTCCATCTCCCTTTCTGTTTCCTGATGGCCTGTCTTCCATAGAGACCCTTCTCACTAAC
ATACAGGGCCTCTTGAAAGTTGCCATAGACAATGCCAGAGCTCAAGAAAAGCAGGTCCAACTGGAAAAAA
CAGAGCTGAAGATGGATTTTTTAAGAGAAAGAGAACTAAGAGAAACACTGGAGAAGCAGCTGGCCATGGA
ACAAAAGAACAGAGCCATAGTTCAAAAGAGGCTAAAGAAGGAAAAGAAAGCAAAGAGAAAACTGCAGGAG
GCACTAGAATTTGAGACAAAACGCCGTGAGCAAGCAGAGCAGACACTGAAACAGGCAGCTTCAGCGGACA
GTCTCCGGGTCTTAAATGACTCCCT
nt 810 - nt 1674 of AJ005669.1
Notes:The Dach1 probe used in this study by Hammond et al., 1998 [PMID:9651501] is described as follows: "Probe was produced using a DIG labelling kit (Boehringer) to transcribe from the T7 promoter of PCR 2.1 containing nucleotides 1674-810 of Dach." Editors Note: The mouse Dach cDNA sequence referred to was deposited in the EMBL database with accession number AJ005669.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Age:10.5 dpc
Theiler Stage:TS17
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Fixation:4% paraformaldehyde
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Hammond KL; Hanson IM; Brown AG; Lettice LA; Hill RE, 1998 [PMID:9651501] , Indexed and Spatially Mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1016/S0925-4773(98)00071-9] [ PMID:9651501] Hammond KL, Hanson IM, Brown AG, Lettice LA, Hill RE 1998 Mammalian and Drosophila dachshund genes are related to the Ski proto-oncogene and are expressed in eye and limb. Mech Dev (74):121-31
Links: Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEMAGE