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EMAGE:4981

Des desmin ( MGI:94885)
TS16 (9.5 dpc)
immunohistochemistry

Data Images
EMAGE:4981
FigSupp2f. Copyright: Reprinted with permission from Cell Press from [doi:10.1016/S0092-8674(00)81010-7] Cell 98(2): 147-157, Carmeliet P; et al, Targeted deficiency or cytosolic truncation of the VE-cadherin gene in mice impairs VEGF-mediated endothelial survival and angiogenesis. Copyright 1999 [PMID:10428027]

Expression pattern clarity: three stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
Image annotations: CA - common atrium; CV - common ventricle.
Expression Pattern Description
Spatial Annotation:
EMAGE:4981Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
4981_wholemount_moderate.wlz
4981_wholemount_weak.wlz
4981_wholemount_notDetected.wlz
4981_wholemount_strong.wlz
4981_wholemount_possible.wlz
(what is wlz format?)
Download all expression domains: EMAGE:4981_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
heart
detected detected
Annotation Validation: EMAGE Editor
Detection Reagent
Type:antibody
Identifier:Des antibodyA
Entity Detected:Des, desmin ( MGI:94885)
Notes:The anti-DES (desmin) antibody used in this study by Carmeliet et al., 1999 [PMID:10428027] is indicated as being obtained from ICN Pharmaceuticals, Costa Mesa, CA, product # 10519. Editors note: ICN Pharmaceuticals = MP Biomedicals. Information from the MP Biomedicals online catalogue is as follows: "DESMIN, ANTI-HUMAN, (CLONE D9), Isotype mouse IgG1. The antibody was prepared from human leyomyoma. The antibody is specific to 53 kDa desmin protein in immunoblotting and gives the same cell staining pattern as cat. no. 10570, polyclonal desmin antibody. No cross-reactivity with any other intermediate filament protein. It does cross-react with desmin of most mammals including mouse, rabbit and rat. It is supplied as clarified cell culture supernatant with 0.09% sodium azide."
Antibody Type:monoclonal
Raised In:mouse
Supplier:MP Biomedicals
Catalogue Number:10519
Specimen
Organism:mouse
Age:9.5 dpc
Theiler Stage:TS16
Mutations:none (wild-type)
Preparation:wholemount
Procedures
General Information
Authors:Carmeliet P; Lampugnani MG; Moons L; Breviario F; Compernolle V; Bono F; Balconi G; Spagnuolo R; Oostuyse B; Dewerchin M; Zanetti A; Angellilo A; Mattot V; Nuyens D; Lutgens E; Clotman F; de Ruiter MC; Gittenberger-de Groot A; Poelmann R; Lupu F; HerbertJM; Collen D; Dejana E, 1999 [PMID:10428027] , Indexed and Spatially Mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1016/S0092-8674(00)81010-7] [ PMID:10428027] Carmeliet P, Lampugnani MG, Moons L, Breviario F, Compernolle V, Bono F, Balconi G, Spagnuolo R, Oostuyse B, Dewerchin M, Zanetti A, Angellilo A, Mattot V, Nuyens D, Lutgens E, Clotman F, de Ruiter MC, Gittenberger-de Groot A, Poelmann R, Lupu F, Herbert JM, Collen D, Dejana E 1999 Targeted deficiency or cytosolic truncation of the VE-cadherin gene in mice impairs VEGF-mediated endothelial survival and angiogenesis. Cell (98):147-57
Links: Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEMAGE