EMAGE mouse gene expression spatial database

EMAGE:4982

Dgcr2 DiGeorge syndrome critical region gene 2 ( MGI:892866)
TS17 (10.5 dpc)
in situ hybridisation

Data Images


Fig2C Copyright: Reprinted with permission from Elsevier from [doi:10.1016/S0925-4773(01)00616-5] Mech Dev 111(1-2): 177-80, Maynard TM; Haskell GT; Bhasin N; Lee JM; Gassman AA; Lieberman JA; LaMantia AS, RanBP1, a velocardiofacial/DiGeorge syndrome candidate gene, is expressed at sites of mesenchymal/epithelial induction. Copyright 2002. [PMID:11804793]	Fig2D Copyright: Reprinted with permission from Elsevier from [doi:10.1016/S0925-4773(01)00616-5] Mech Dev 111(1-2): 177-80, Maynard TM; Haskell GT; Bhasin N; Lee JM; Gassman AA; Lieberman JA; LaMantia AS, RanBP1, a velocardiofacial/DiGeorge syndrome candidate gene, is expressed at sites of mesenchymal/epithelial induction. Copyright 2002. [PMID:11804793]

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Notes:

The embryo in Fig2C is treated with antisense probe and Fig2D shows a sense control.

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

wholemount mapping

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Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

embryo

detected

Annotation Validation:

EMAGE Editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	Dgcr2 probeA
Entity Detected:	Dgcr2, DiGeorge syndrome critical region gene 2 ( MGI:892866)
Sequence:	sense strand is shown >Dgcr2 probeA CATCCTCTCGCTGCTGCTTTTCATGGTCCACCGGCTACGCCAGCGGCGCCGGGAGCGCATCGAGTCCCTG ATTGGAGCAAACTTGCACCATTTCAACCTTGGCCGGAGGATCCCTGGCTTTGACTATGGTCCAGATGGGT TTGGCACAGGCCTCACGCCACTACATCTTTCTGACGACGGGGAAGGTGGGACTTTCCATTTCCACGACCC TCCACCTCCTTATACAGCTTACAAGTACCCAGACATGGACCAGCCGGATGATCCACCACCACCCTATGAG GCGTCTATCAACCCAGACAGTGTGTTCTACGACCCTGCAGATGACGATGCCTTTGAGCCTGTGGAGGCCA GCCTGCCAGCCCCAAGAGATGGTGGCATTGAAGGTGCATTGCCCAGGCACTTGGATCAGCCGCTGCCCCC TGCAGAGACATCCTTAGCAGATCTGGAAGACTCTACAGACAGTAGCAGTGCCCTGCTGGTGCCTCCTGAT CCCGCCCAGAGTGGAAGCACCCCAGCTACAGAAGCACCGCCAGGGGG
	nt 1338 - nt 1874 of NM_010048.3
Notes:	The Dgcr2 probe used in this study by Maynard et al., 2002 [PMID:11804793] is described as follows: "the 537 base DGCR2 amplicon (5'-catcctctcgctgctgcttttcat-3' and 5'-ccccctggcggtgcttctgta-3') was cloned into pBluescript II, and digoxigenin labeled probes (Roche) were made using T3 and T7 RNA polymerase (Promega)."
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Procedures

General Information

Authors:	Maynard TM; Haskell GT; Bhasin N; Lee JM; Gassman AA; Lieberman JA; LaMantia AS, 2002 [PMID:11804793] , Indexed and Spatially Mapped by EMAGE.
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	non-screen
References:	[ doi:10.1016/S0925-4773(01)00616-5] [ PMID:11804793] Maynard TM, Haskell GT, Bhasin N, Lee JM, Gassman AA, Lieberman JA, LaMantia AS 2002 RanBP1, a velocardiofacial/DiGeorge syndrome candidate gene, is expressed at sites of mesenchymal/epithelial induction. Mech Dev (111):177-80
Links:	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source