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EMAGE:4983

Dgcr8 DiGeorge syndrome critical region gene 8 ( MGI:2151114)
TS19 (11 dpc)
in situ hybridisation

Data Images
EMAGE:4983 EMAGE:4983
Fig3F. [doi:10.1016/S0006-291X(03)00554-0] Reprinted from Biochem Biophys Res Comm, vol 304, Shiohama A; Sasaki T; Noda S; Minoshima S; Shimizu N, "Molecular cloning and expression analysis of a novel gene DGCR8 located in the DiGeorge syndrome chromosomal region.", pp 184-90, Copyright (2003) with permission from Elsevier. [PMID:12705904] Fig3G. [doi:10.1016/S0006-291X(03)00554-0] Reprinted from Biochem Biophys Res Comm, vol 304, Shiohama A; Sasaki T; Noda S; Minoshima S; Shimizu N, "Molecular cloning and expression analysis of a novel gene DGCR8 located in the DiGeorge syndrome chromosomal region.", pp 184-90, Copyright (2003) with permission from Elsevier. [PMID:12705904]

Expression pattern clarity: three stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
Fig3G is a control embryo treated with the sense probe. Image annotations: primary brain (b), pharyngeal arch first region (1pa), and limb buds (lb).
Expression Pattern Description
Spatial Annotation:
EMAGE:4983Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
4983_wholemount_moderate_3D_1.wlz
4983_wholemount_weak_3D_1.wlz
4983_wholemount_notDetected_3D_1.wlz
4983_wholemount_strong_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:4983_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Text Annotation:
StructureLevelPatternNotes
1st branchial arch
detected detected
regionalwhole-mount in situ hybridization exhibited this Dgcr8 expression in the pharyngeal arch first region including palate precursors.
limb
detected detected
the embryos of this stage showed signals in the developing limb bud.
brain
detected detected
regionalthe embryos of this stage showed signals in the neuroepithelium of primary brain
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:Dgcr8 probeA
Entity Detected:Dgcr8, DiGeorge syndrome critical region gene 8 ( MGI:2151114)
Sequence:sense strand is shown

>Dgcr8 probeA
TCGGCAGAGGGGTGGTGTAAGAATAAAAGAGTTGGGAAACAATTAGCATCTCAGAAAATCCTTCAGCTAC
TGCACCCACATGTCAAGAACTGGGGTTCCTTACTACGCATGTATGGTCGTGAGAGCAGCAAAATGGTCAA
GCAGGAGACCTCTGACAAGAGTGTGATAGAGCTACAGCAGTATGCCAAGAAGAACAGGCCCAACCTTCAC
ATCCTGAGCAAGCTACAAGAAGAGATGAAGAGGCTGGCTGCAGAGCGGGAGGAGACTCGGAAGAAACCCA
AGATGTCAATTGTAGCATCTGCCCAGCCTGGTGGTGAGCCCTTGTGCACAGTCGATGTATGAGGTAGGCA
GCATGGGCCAACAGTGCTACCCAGGAGAGACCATCAGCCACACATCATCACTCTGCAGCTCCAGGCCTCC
AACCTAAGTTCCTTCCCTGTGGCAGGGTCTGGGCTCTGGCTCTGGCACATGGGGACAGCTGTGACTACAC
AGTACACATGCAAAGAAGCAGTTCTGGACAAGCTGCTCTCAATGTGACTGGATATACTTAAGGATCAGTC
ATAAGT
Notes:The Dgcr8 probe used in ths study by Shiohama et al., 2003 [PMID:12705904] is described as follows: "In situ hybridization was performed using digoxigenin labeled antisense riboprobes synthesized from the mouse cDNA clone Dgcr8. The cDNA clone was obtained from I.M.A.G.E. clones (Accession No. AA217940), linearized with restriction endonucleases, and riboprobe was synthesized by T3, T7 RNA polymerase (Ampliscribe T3/T7 High Yield Transcription Kits, Epicentre Technologies, Madison, WI)."
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Age:11 dpc
Theiler Stage:TS19
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Shiohama A; Sasaki T; Noda S; Minoshima S; Shimizu N, 2003 [PMID:12705904] , Indexed and Spatially Mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1016/S0006-291X(03)00554-0] [ PMID:12705904] Shiohama A, Sasaki T, Noda S, Minoshima S, Shimizu N 2003 Molecular cloning and expression analysis of a novel gene DGCR8 located in the DiGeorge syndrome chromosomal region. Biochem Biophys Res Commun (304):184-90
Links: Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEMAGE