EMAGE mouse gene expression spatial database

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EMAGE:4985

Wnt9b wingless-type MMTV integration site 9B ( MGI:1197020)
TS21 (13.5 dpc)
in situ hybridisation

Data Images


Raw signal output, as movie.	All sections along the X-axis, as movie.	All sections along the Y-axis, as movie.	All sections along the Z-axis, as movie.	Photograph prior to 3D imaging.

Photograph prior to 3D imaging.	Photograph prior to 3D imaging.	Photograph prior to 3D imaging.	Sample morphology following 3D imaging, as movie

View 3D opt image:
Download 3D images in woolz format
Expression pattern clarity:

Notes:

This is a traditionally produced wholemount in situ hybridisation stained using alkaline phosphatase + BM Purple. It has been imaged using Optical Projection Tomography. The visible channel depicting the signal has been false-coloured green and the fluorescence channel depicting autofluorescence has been false-coloured blue.

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

palatal shelf epithelium

weak

homogeneous

primary palate epithelium

possible

upper lip

moderate

graded

Annotation Validation:

EMAGE Editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	Wnt9b_cloneB
Entity Detected:	Wnt9b, wingless-type MMTV integration site 9B ( MGI:1197020)
Sequence:	sense strand is shown >Wnt9b_cloneB TTCCAGGCCCAGAGACGGAGGCAGGTTGGTCAAAGCCAGTCACTCTCTGAACCCAGAGGTTGAGGAAGAG TGCATGCTGCGTGGAACGCTGGTCTTCCCCCATGGATGGCATGCTAGTTTCTCCAGCAAGCTGAGTCTCA TGTCCCCAAAGACGGGGACTTCCTGAGAAGCCTGGAGAGACAAGGGCTCCGTGGATGTCACTCTTAGGGA GGGTGTCCTGCAGCCCTCATTGACCTCCACGACTAGGCTATGGTCTCCAGCCCCTCACAGCTCGTGGATA ATTTGTGTTTCTTCGCTTTTGTTTTTTGTCTTTTCAAAGTGACTTTTTCCCCACTGGATTTCTAAGTTTC TCTTTGAAAATCAGTTCACTGGCAAATGGGACCTGCATCCTGACCTGGCTGCCTGCATCAGGAGCGACAC CAAACAGAGTGCGTGGGGATCCCCAATTGGCCCAGTGTCCCCCGGCCCTTCCTTAAGTCACACAAGCTCC CGTGTGGCTTTCGTGAGCATGGAGAACCTGTCCCCTGGTCTTAGAGAAAGCCAGCCATTCTGCCACCCTC TGTTTGTCTGGCAGACAGATTACCACACCGTGGCTGTCTTTCTAGCCAAAGCTTCCTCTCTCAACACCCA TGAACGTCCATGCTTCCTGTCTGAGCACTGAGGAGAACCCCAGCGGAGCTCATTGTTCAGTGCTGGAATA CCCATCCCCCCTCCCGTTGATTATTTAGGGAGTGTCTGATAATGCCAGGGGATACTCTGGGTGCTAGGGC GCAGAAGTACTTAAGAGCAAGTCCCAGCCTCAGGGGACTTATATGCCGGCGAGGAGAAAGCCAACAAACC AATAAACTATGCAC
	nt 3663 - nt 4516 of NM_011719.3
Notes:	A Wnt9b probe template fragment in pSPORT1 was kindly provided by Andrew Lidral (Lan et al., 2006). To generate Wnt9b antisense probe, the plasmid was cut with Sal I and labelled probe transcribed from the SP6 promoter. Template clone available on request. Probe length: 854nt
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	CD-1
Age:	13.5 dpc
Theiler Stage:	TS21
Mutations:	none (wild-type)
Preparation:	opt

Procedures

Fixation:	4% paraformaldehyde
Embedding:	agarose
Staining procedure:	alkaline phosphatase + BMpurple

General Information

Authors:	Bozena Palinkasova, Mike Dixon
Principal investigator:	Mike Dixon, University of Manchester, Faculty of Life Sciences Michael Smith Building Oxford Road, Manchester, England M13 9PT
Submitted by:	Bozena Palinkasova, University of Manchester, Faculty of Life Sciences Michael Smith Building Oxford Road, Manchester, England M13 9PT
Experiment type:	screen
References:	[ doi:10.1002/dvdy.20723] [ PMID:16496313] Lan Y, Ryan RC, Zhang Z, Bullard SA, Bush JO, Maltby KM, Lidral AC, Jiang R 2006 Expression of Wnt9b and activation of canonical Wnt signaling during midfacial morphogenesis in mice. Dev Dyn (235):1448-54
Links:	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source