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EMAGE:4987

Wnt9b wingless-type MMTV integration site 9B ( MGI:1197020)
TS17 (10.5 dpc)
in situ hybridisation

Data Images
EMAGE:4987 EMAGE:4987 EMAGE:4987 EMAGE:4987 EMAGE:4987
Raw signal output, as movie. All sections along the X-axis, as movie. All sections along the Y-axis, as movie. All sections along the Z-axis, as movie. Photograph prior to 3D imaging.
EMAGE:4987 EMAGE:4987 EMAGE:4987
Photograph prior to 3D imaging. Photograph prior to 3D imaging. Sample morphology following 3D imaging, as movie

View 3D opt image: EMAGE genex expression entry
Download 3D images in woolz format
Expression pattern clarity: three stars
Notes:
This is a traditionally produced wholemount in situ hybridisation stained using alkaline phosphatase + BM Purple. It has been imaged using Optical Projection Tomography. The visible channel depicting the signal has been false-coloured green and the fluorescence channel depicting autofluorescence has been false-coloured blue.
Expression Pattern Description
Text Annotation:
StructureLevelPatternNotes
latero-nasal process
strong strong
regional
medial-nasal process
strong strong
regional
frontal process
not detected not detected
homogeneous
1st branchial arch mandibular component
strong strong
regional
1st branchial arch maxillary component
strong strong
regional
2nd branchial arch
strong strong
regional
3rd branchial arch
strong strong
graded
forelimb bud apical ectodermal ridge
strong strong
homogeneous
hindlimb bud apical ectodermal ridge
strong strong
homogeneous
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:Wnt9b_cloneB
Entity Detected:Wnt9b, wingless-type MMTV integration site 9B ( MGI:1197020)
Sequence:sense strand is shown

>Wnt9b_cloneB
TTCCAGGCCCAGAGACGGAGGCAGGTTGGTCAAAGCCAGTCACTCTCTGAACCCAGAGGTTGAGGAAGAG
TGCATGCTGCGTGGAACGCTGGTCTTCCCCCATGGATGGCATGCTAGTTTCTCCAGCAAGCTGAGTCTCA
TGTCCCCAAAGACGGGGACTTCCTGAGAAGCCTGGAGAGACAAGGGCTCCGTGGATGTCACTCTTAGGGA
GGGTGTCCTGCAGCCCTCATTGACCTCCACGACTAGGCTATGGTCTCCAGCCCCTCACAGCTCGTGGATA
ATTTGTGTTTCTTCGCTTTTGTTTTTTGTCTTTTCAAAGTGACTTTTTCCCCACTGGATTTCTAAGTTTC
TCTTTGAAAATCAGTTCACTGGCAAATGGGACCTGCATCCTGACCTGGCTGCCTGCATCAGGAGCGACAC
CAAACAGAGTGCGTGGGGATCCCCAATTGGCCCAGTGTCCCCCGGCCCTTCCTTAAGTCACACAAGCTCC
CGTGTGGCTTTCGTGAGCATGGAGAACCTGTCCCCTGGTCTTAGAGAAAGCCAGCCATTCTGCCACCCTC
TGTTTGTCTGGCAGACAGATTACCACACCGTGGCTGTCTTTCTAGCCAAAGCTTCCTCTCTCAACACCCA
TGAACGTCCATGCTTCCTGTCTGAGCACTGAGGAGAACCCCAGCGGAGCTCATTGTTCAGTGCTGGAATA
CCCATCCCCCCTCCCGTTGATTATTTAGGGAGTGTCTGATAATGCCAGGGGATACTCTGGGTGCTAGGGC
GCAGAAGTACTTAAGAGCAAGTCCCAGCCTCAGGGGACTTATATGCCGGCGAGGAGAAAGCCAACAAACC
AATAAACTATGCAC
nt 3663 - nt 4516 of NM_011719.3
Notes:A Wnt9b probe template fragment in pSPORT1 was kindly provided by Andrew Lidral (Lan et al., 2006). To generate Wnt9b antisense probe, the plasmid was cut with Sal I and labelled probe transcribed from the SP6 promoter. Template clone available on request. Probe length: 854nt
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:CD-1
Age:10.5 dpc
Theiler Stage:TS17
Mutations:none (wild-type)
Preparation:opt
Procedures
Fixation:4% paraformaldehyde
Embedding:agarose
Staining procedure:alkaline phosphatase + BMpurple
General Information
Authors:Bozena Palinkasova, Mike Dixon
Principal investigator:Mike Dixon, University of Manchester, Faculty of Life Sciences Michael Smith Building Oxford Road, Manchester, England M13 9PT
Submitted by:Bozena Palinkasova, University of Manchester, Faculty of Life Sciences Michael Smith Building Oxford Road, Manchester, England M13 9PT
Experiment type:screen
References:[ doi:10.1002/dvdy.20723] [ PMID:16496313] Lan Y, Ryan RC, Zhang Z, Bullard SA, Bush JO, Maltby KM, Lidral AC, Jiang R 2006 Expression of Wnt9b and activation of canonical Wnt signaling during midfacial morphogenesis in mice. Dev Dyn (235):1448-54
Links: Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceFACEBASE