| Type: | in situ hybridisation probe |
| Identifier: | Dgkz probeA |
| Entity Detected: | Dgkz, diacylglycerol kinase zeta ( MGI:1278339) |
| Notes: | The Dgkz probe used in this study by Ding et al., 1998 [PMID:9657393] is one of two possible probes described as follows: "The first probe was a 716-bp 3' PstI/EcoRI fragment. It was subcloned into pBluescript II and used to make digoxigenin-labeled RNA probe by in vitro transcription (Boehringer Mannheim). The second one was from the catalytic domain region of the mDGK-zeta gene. A pair of primers with T7 or T3 promoters attached was used to amplify a 558-bp mDGK-zeta cDNA fragment. Amplified PCR products were used as templates to make digoxigenin-labeled RNA probes by using an in vitro transcription kit (Boehringer Mannheim). The sequences of the primers were: 5'-CAGAGATGCAattaaccctcactaaagggaGAGTCTCGAGAAGCCAACCCAGAG-3', T3 and 5'-CCAAGCTTCtaatacgactcactatagggagaCCCTGCTCACCTGGATCCTCAG-3', T7."
Editors Note: The T3 and T7 promotors are denoted here in the primer sequences in lower case. |
| Chemistry: | RNA |
| Strand: | antisense |
| Label: | digoxigenin |
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