| Type: | in situ hybridisation probe |
| Identifier: | Dido1 probeA |
| Entity Detected: | Dido1, death inducer-obliterator 1 ( MGI:1344352) |
| Sequence: | sense strand is shown
>Dido1 probeA
AGATCTGTGGGTGCTGATGGCACAGACTGCACAAGCATAGGGACAGTAGAGCAGAAGTCCGGAGAAGACC
AGGGCATAAAGGGTAGGATTGAGAAGGCAGCAAACCCCAGCGGCAAGAAAAAACTCAAGATATTCCAGCC
TGTCGTAGAGGCTCCTGGTGCTCCTAAATGCATTGGCCCTGGGTGTTCCAGTGTAGCACAGCCTGACTCT
GTGTATTGCAGTAATGACTGCATTCTCAAACACGCAGCAGCTACCATGAGATTTCTAAGTTCAGGTAAAG
AACAAAAAACAAAACCCAAGGAAAAGGTCAAGACGAAGCCAGAAAAGTTCAGTCTTCCAAAATGCAGTGT
TCAGGTGGGGATTAAAATCTCTTCTGTGCACAAGAGACTAGCGTCAGAGAAAAGGGAAAACCCAGTGAAG
AAAGTGATGCTGGCTTCCAGGAGTGAGACTTCTGGGAAGGAGGCAGCCTGTGAGAGCAGCACACCATCCT
GGGCAAGTGACCACAACTACAATGCTGTGAAGCCAGAGAAGCCAGAGAAGCCCACTGCACTCTCGCCCAC
CCTATTGAGTAAATGTACGTATCACCCAAAGGCTGGCTTCCCAGGCCCCTCCCATCATCTGGGTGGCTGC
CTGGGGCTGTCTAGGACCAGAGTCCTGGGTGTTCTGGTGCTGATAGTAGCCAGCAGCTCACTGCCAGCCA
GAAGCAGATACCAAGATGCCTCTGGACCCCAGGTGTTCCTGCCTAGCCTGTGGAGCCTCTCTGGGTGGTT
CCTAAAGAGCTGTGTAGGCCTCATGTTGGAGGCAATTTCTTATTTCAGTTTTAGGCCTTGGTAAGTATTT
GAACTGCTCTCAACAAGATGGGACAACATCAGTGCACTGTTCAGACTCAGTTCAGACTTGAGTTCCTCAC
AGGACAGCCCAGCACAAACCATGGAGTTTTCCCACGTTACCTATTTTTCTTCAACATGGTCACCACTGCT
CTTCTATAGAAAAGTGATTTTTTTCATGAGTTAGGGCATTTTTGAAAGGACAGGTAGACAATTTGTGTCT
ATTTCACATACTACACACCTACATAAACAGGTTTGAATTTTGAAGGTCATCTGCTGCTCAGATCCCATGG
TGAGTAATCGTGCTGACCATACACCTACAGCAGTATTCTCATCAAAGTGAGAATTGTGGGTGGTAGCTTC
CAGACTCGTAGATGCCCCAGATTTCAAATCAGTGTTTATAGTTTGAGTAAACTTTTGAAACCCAAGATTA
GATCACTGAGTTTGGAGCCATTCTTGTTTCACTGCTGTACAAGGTACACCTTTCTTACTTTTTGTACAGT
GGTTTCAAGTGAATTTTCATCTGTGTTCTC
|
| | nt 1243 - nt 2602 of AJ238332.1 |
| Notes: | The Dido1 (DIO-1) probe used in this study by Garcia-Domingo et al., 1999 [PMID:10393935] is described as follows: "The DIO-1 digoxigenin probe was made by BglII digestion of the DIO-1pGEM-T and transcription from the SP6 promoter". Construction of DIO-1pGEM-T is described as: "DIO-1 cDNA was obtained from WOL-1 cDNA by 5' rapid amplification of cDNA ends (RACE) by using a Marathon cDNA Amplification Kit (CLONTECH), with the 3' primer L282 (5' -AGGTGTACCTTGTACAGCAGTGAAAC- 3'). The resulting 2.6-kbp band was excised from the gel and cloned in the TA-type vector pGEM-T (Promega). Resulting clones were sequence analyzed for orientation, and the oriented sense with respect to the T7 promoter was called DIO-1pGEM-T."
Editors note: The authors state elsewhere "The sequence reported in this paper has been deposited in the GenBank database (accession no. AJ238332)." As AJ238332.1 is 2.6kb in length, I have assumed that this is the same sequence as the 2.6kb insert sequence of DIO-1pGEM-T. BglII cuts this sequence once, at position 1243. |
| Chemistry: | RNA |
| Strand: | antisense |
| Label: | digoxigenin |
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