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EMAGE:500

Mesp2 mesoderm posterior 2 ( MGI:1096325)
TS15 (9.5 dpc)
in situ hybridisation

Data Images
EMAGE:500
Fig 7Aabcd Koizumi et al, 2001 [PMID:11262239] . Copyright: This image is from Development and is displayed with the permission of the Company of Biologists Ltd who owns the copyright.

Expression pattern clarity: three stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
Tha spatial mapping in this EMAGE submission is based on all of these images and has been done at low resolution to solely indicate expression of Mesp2 in the presomitic mesoderm.
Expression Pattern Description
Spatial Annotation:
EMAGE:500Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
500_wholemount_moderate_3D_1.wlz
500_wholemount_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:500_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Text Annotation:
StructureLevelPatternNotes
tail unsegmented mesenchyme
moderate moderate
regionalThe expression first appears one segement in width and is then rapidly down-regulated in the caudal half of the somite
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:1932050
Entity Detected:Mesp2, mesoderm posterior 2 ( MGI:1096325)
Sequence:sense strand is shown

>MGI:1932050
GGATCCCCGGGTGACACCTCCTTATTGTCCCCAAATACAGTCACCCTTACACCAGTCCCTAGAAAGAGCC
GCTGACTCCTCTCCCTGGGCACCACCTCAAGCATGTCCTGGCATGCAGATGTCCCCAGAGCCTAGGAACA
AGACTGGACACTGGACACAATCCACTGAACCTGCAGAGCTGACTAAAGTGTATCAGAGTCTTTCTGTGTC
TCCAGAACCCCGCCTGTCCCTGGGAAGCCCACTTCTCCTGCCCCGCCCATCATGCCAGAGACTACAGCCT
CAGCCTCAGCCTCAGCCTCAGTGGGGCTGCTGGGGCCACGATGCAGAGGTGCTCTCCACCTCTGAGGATC
AGGGTTCCAGCCCTGCCCTCCAGCTTCCTGTGGCCAGCCCCACCCCCAGCTCAGGCCTGCAGCTCAGTGG
CTGTCCTGAACTTTGGCAGGAAGACCTGGAAGGACCCCCACTGAATATTTTCTACTAATATGGCCTTACC
TGCACCTGCGGTGTGGATTTGGGCTGCCTTGGAAGTGCCTTTATCTGCCTCTTCTGTGACATTTTGAGGT
CTGCTTGGCTATGGCCCCCCTTTCCCAGATGACCCCTTTCATGGAAACGGTGTCTGGGGCAGATAAACAT
TGGGTACCTTCACTGTTTTGAGGGGGGCTGAGACAAGACACCCCCGCTTCTAGGTATCTTCTAGAGCAGT
GATTCTCAACCTTGCTAATGCCGTGACCCTTTAATACATACAGTTCCTCATGGTGTGGTGACCCCCCCCA
ACCATAAAATTATCTTATTGCTACTTCATAACTGTAATTTTGCTACTGTTATAAATCATAATGTAAATCT
CTGTGTTTTCTGGTAGTTTTAGGTAACCCCTGTGAGAGGGTCGTTCCATACTCCACCCCCCAGGGGTCAC
AACCTACAGGCTGAGAACTACTATTCTAAAGGCTCAGCACTCTGCAGAGCACCAGGCTGAGCTGCTCTGA
GTACGGGGTGGCCTGAGTGTGGAGGACAAAGGAGGTCCATGTCCTGCTTGCTGGAGACAGCTGGCCAGAA
TCGTATATGCTGGTTTTATAACTGTTTTATTATTAAAGAGAAGAAAACCAAAAAAAAAAAAAAAAAAA
nt 843 - nt 1960 of U71125.1
Notes:The probe used in this study by Koizumi et al, 2001 [PMID:11262239] is indicated as that used by Saga et al, 1997 [PMID:9242490] ie. "cDNA clone linearized with BamHI site within exon 1 to remove the bHLH region".
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Age:9.5 dpc
Theiler Stage:TS15
Mutations:none (wild-type)
Preparation:wholemount
Procedures
General Information
Authors:Koizumi et al, 2001 [PMID:11262239] Indexed by GXD, Spatially mapped by EMAGE
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ PMID:11262239] Koizumi K, Nakajima M, Yuasa S, Saga Y, Sakai T, Kuriyama T, Shirasawa T, Koseki H 2001 The role of presenilin 1 during somite segmentation. Development (128):1391-402
 [ doi:10.1101/gad.11.14.1827] [ PMID:9242490] Saga Y, Hata N, Koseki H, Taketo MM 1997 Mesp2: a novel mouse gene expressed in the presegmented mesoderm and essential for segmentation initiation. Genes Dev (11):1827-39
Links:MGI:1932862 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI