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EMAGE:5000

Trp63 transformation related protein 63 ( MGI:1330810)
TS17 (10.5 dpc)
in situ hybridisation

Data Images
EMAGE:5000 EMAGE:5000 EMAGE:5000 EMAGE:5000 EMAGE:5000
Raw signal output, as movie. All sections along the X-axis, as movie. All sections along the Y-axis, as movie. All sections along the Z-axis, as movie. Photograph prior to 3D imaging.
EMAGE:5000 EMAGE:5000
Photograph prior to 3D imaging. Sample morphology following 3D imaging, as movie

View 3D opt image: EMAGE genex expression entry
Download 3D images in woolz format
Expression pattern clarity: three stars
Notes:
This is a traditionally produced wholemount in situ hybridisation stained using alkaline phosphatase + NBT/BCIP. It has been imaged using Optical Projection Tomography. The visible channel depicting the signal has been false-coloured green and the fluorescence channel depicting autofluorescence has been false-coloured blue.
Expression Pattern Description
Text Annotation:
StructureLevelPatternNotes
forelimb bud apical ectodermal ridge
strong strong
homogeneous
hindlimb bud apical ectodermal ridge
strong strong
homogeneous
latero-nasal process
strong strong
regional
medial-nasal process
strong strong
regional
1st branchial arch mandibular component
strong strong
regional
1st branchial arch maxillary component
strong strong
regional
2nd branchial groove
moderate moderate
1st branchial arch mandibular component ectoderm
strong strong
regional
1st branchial arch maxillary component ectoderm
strong strong
regional
hindlimb bud ectoderm
moderate moderate
regional
forelimb bud ectoderm
moderate moderate
regional
Annotation Validation: Submitter + EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:Trp63_cloneA
Entity Detected:Trp63, transformation related protein 63 ( MGI:1330810)
Sequence:sense strand is shown

>Trp63_cloneA
TTTCATGTGTAACAGCAGCTGCGTCGGAGGAATGAACAGACGTCCAATTTTAATCATCGTTACTCTGGAA
ACCAGAGATGGGCAAGTCCTGGGCCGACGGTGCTTTGAGGCCCGGATCTGTGCTTGCCCAGGAAGAGACC
GGAAGGCAGATGAAGACAGCATCAGAAAGCAGCAAGTATCGGACAGCGCAAAGAACGGCGATGGTACGAA
GCGCCCTTTCCGTCAGAATACACACGGAATCCAGATGACTTCCATCAAGAAACGGAGATCCCCAGATGAT
GAGCTGCTGTACCTACCAGTGAGAGGTCGTGAGACGTACGAGATGTTGCTGAAGATCAAAGAGTCACTGG
AGCTCATGCAGTACCTCCCTCAGCACACGATCGAAACGTACAGGCAGCAGCAGCAGCAGCAGCACCAGCA
CCTACTTCAGAAACATCTCCTTTCAGCCTGCTTCAGGAATGAGCTTGTGGAGCCCCGGGGAGAAGCTCCG
ACACAGTCTGACGTCTTCTTTAGACATTCCAACCCCCCAAACCACTCCGTGTACCCATAGGTCCCCAGCT
ATGTGTTTGAGTTCATGTGCTTGTTGTGTTTCTGTGTGCGTTTGTGTATATGCACATGCGTGTTAGTGTT
TCCAGCCCTCACAAACAGGACTTGAAGACATTTTGGCTCAGAGACCCAGCTGCTCAAAGGCACACATCCA
CTAGTGAGAGAATCTTTGAAGGGACTCAAAATTTTACAAAGCAGAGATGCTTTCTGCACATTTTGTATCT
TTAGATCCTGCCTTGGTTGGACGGGAGCCGCGACTGTGCTTGTCTGTGAGCTTTCTATTGTTTTCCCAGG
AGGGAGGGGGAATCCATTGGGAAAGAGGCATTGCAAAGTTTATTGGAAACCTTTTCTGTTACCTCCTGTT
GTGTTTCTAAAACTCATAATAAAGCTTTTGAGTAGGTCTCAAA
nt 1729 - nt 777 of BC092537.1
Notes:This probe was produced from a Trp63 probe template fragment (~1.5 kb) in pBluescriptSK-. To produce antisense probe the template was cut with EcoRI and transcribed with the T7 polymerase. Probe available on request. Probe length: 953nt
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:CD-1
Age:10.5 dpc
Theiler Stage:TS17
Mutations:none (wild-type)
Preparation:opt
Procedures
Fixation:4% paraformaldehyde
Embedding:agarose
Staining procedure:alkaline phosphatase + BMpurple
General Information
Authors:Laura Ward, Mike Dixon
Principal investigator:Mike Dixon, University of Manchester, Faculty of Life Sciences Michael Smith Building Oxford Road, Manchester, England M13 9PT
Submitted by:Laura Ward, University of Manchester, Faculty of Life Sciences Michael Smith Building Oxford Road, Manchester, England M13 9PT
Experiment type:screen
Links: Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceFACEBASE