EMAGE mouse gene expression spatial database

EMAGE:5001

Dlg4 discs, large homolog 4 (Drosophila) ( MGI:1277959)
TS15 (8.5 dpc)
in situ reporter

Data Images


Fig7, E10.5, PSD-95. [doi:10.1111/j.1460-9568.2005.03874.x] Eur J Neurosci 2005 21(2): 351-62, Porter K; Komiyama NH; Vitalis T; Kind PC; Grant SG, Differential expression of two NMDA receptor interacting proteins, PSD-95 and SynGAP during mouse development. Copyright 2005 [PMID:15673435]

Expression pattern clarity:
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Expression Pattern Description

Spatial Annotation:

Annotation colour key:

wholemount mapping

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Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

notochord

strong

surface ectoderm

strong

Authors state that expression is high in 'skin' at this stage.

trunk somite

not detected

homogeneous

Annotation Validation:

EMAGE Editor

Detection Reagent

Type:	in situ reporter
Entity Detected:	Dlg4, discs, large homolog 4 (Drosophila) ( MGI:1277959)
Notes:	Dlg4 (PSD-95) expression was detected in this study by Porter et al., 2005 [PMID:15673435] using a targeted lacZ reporter line, whereby a stop codon was introduced into sequence encoding the PDZ3 domain. In addition, a cassette containing IRES-lacZ and neo was inserted downstream to replace sequence putatively essential for subcellular localization. An RNAse protection verified transcription of the targeted allele. While low levels of the mutant protein containing the PDZ1 and PDZ2 domains were detected by Western blot analysis of homozygous mutant forebrain extracts, the mutant protein was undetected in the synaptosome, synaptic plasma membrane, and posynaptic density fractions. Comparisons with previous results of in situ hybridization studies showed the expression pattern of beta-galactosidase to resemble that of the endogenous gene.
Labelled with:	LacZ

Specimen

Procedures

Fixation:

1% formaldehyde/0.9% gluteraldehyde

General Information

Authors:	Porter K; Komiyama NH; Vitalis T; Kind PC; Grant SG, 2005 [PMID:15673435] , Indexed and Spatially Mapped by EMAGE.
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	non-screen
References:	[ doi:10.1111/j.1460-9568.2005.03874.x] [ PMID:15673435] Porter K, Komiyama NH, Vitalis T, Kind PC, Grant SG 2005 Differential expression of two NMDA receptor interacting proteins, PSD-95 and SynGAP during mouse development. Eur J Neurosci (21):351-62
Links:	MGI:2182460 allele description
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source