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EMAGE:5008

Dnm3os dynamin 3, opposite strand ( MGI:3052332)
TS15 (9.5 dpc)
in situ hybridisation

Data Images
EMAGE:5008
Fig4a. from [doi:10.1016/j.ygeno.2005.02.001] Genomics 2005 85(6): 782-789, Loebel DA; Tsoi B; Wong N; Tam PP, A conserved noncoding intronic transcript at the mouse Dnm3 locus. Copyright 2005 [PMID:15885504]

Expression pattern clarity: three stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:5008Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
5008_wholemount_moderate_3D_1.wlz
5008_wholemount_weak_3D_1.wlz
5008_wholemount_notDetected_3D_1.wlz
5008_wholemount_strong_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:5008_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: three stars
Text Annotation:
StructureLevelPatternNotes
1st branchial arch
detected detected
2nd branchial arch
detected detected
forelimb bud
detected detected
trunk somite
detected detected
olfactory placode
detected detected
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:3587750
Entity Detected:Dnm3os, dynamin 3, opposite strand ( MGI:3052332)
Sequence:sense strand is shown

>MGI:3587750
TGTGAGAGTTGAAAAATCTTAATTATTTTTAAAAAAAAGAGTTGAGATTATATATATATCCACACATACA
TATCCCTTTGTTTGCACAGGAAATTAAAAGCACTATTAGTTCACTTATGGAAGTTGTGTGTCTCAGGAAA
CACACTTTCTTGACTAACGCGTCACGAAAGAGGACAGAGAAGAATCACTTGGATCATATTTAAGATTTCT
CTAATTTTGAGGGCCTATTGTATACTAGAAATAATTGAATTTTATAACTTGCCTTTATTTAATGATATTT
AATTTAAAATTTTCTCTTCATATTAAATGCATTTTTTTTAATTTCAAGAAGAATAACCTTTCTTCACAGG
TCCCTTCCCTATTTCTCCCATGGGACAGTTTTTAGTCTGAAGATACAAAGGGCATAAGGGTGATTCTCCT
GTAAGTTCAACAGTAAAAAGTAACAATTTCTTTGTAAACCCGGAGTCTCTGCCTGTTCTTGGTTGGAGTT
CTAACACACCATTTTCAAGGAAAATTCTAAATGAATGAAGGAGTGTGGTACTTAATTTTAAATGTATGAG
TTATATTAAAAGTACAAAAAAGTTGTTTGATAACCCTCTGCTGTATGAAGACATGGTGTTAAGCCTGTGT
GCTGGTGGATATATCCCTTATAGACACTTTAGGATAAAAATAAAGATATCGATTCC
Notes:The Dnm3os probe used in this study by Loebel et al., 2005 [PMID:15885504] is described as follows. "Digoxigenin-labeled probes were prepared using Ampliscribe (Epicentre) from linearized DNA of clone M24 (Loebel et al., 2002 [PMID:12124942] )." Editors Note: BB043240 is a sequence identifier for clone M24 given in Table 1 of Loebel et al., 2002 [PMID:12124942] .
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:ARC/S
Age:9.5 dpc
Theiler Stage:TS15
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Fixation:4% paraformaldehyde
Staining procedure:alkaline phosphatase + unspecified
General Information
Authors:Loebel DA; Tsoi B; Wong N; Tam PP, 2005 [PMID:15885504] , Indexed by GXD, Spatially Mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1016/j.ygeno.2005.02.001] [ PMID:15885504] Loebel DA, Tsoi B, Wong N, Tam PP 2005 A conserved noncoding intronic transcript at the mouse Dnm3 locus. Genomics (85):782-9
 [ doi:10.1002/gene.10091] [ PMID:12124942] Loebel DA, O'Rourke MP, Steiner KA, Banyer J, Tam PP 2002 Isolation of differentially expressed genes from wild-type and Twist mutant mouse limb buds. Genesis (33):103-13
Links:MGI:3587779 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI