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EMAGE:5010

Dnm3os dynamin 3, opposite strand ( MGI:3052332)
TS19 (11.5 dpc)
in situ hybridisation

Data Images
EMAGE:5010
Fig4f. from [doi:10.1016/j.ygeno.2005.02.001] Genomics 2005 85(6): 782-789, Loebel DA; Tsoi B; Wong N; Tam PP, A conserved noncoding intronic transcript at the mouse Dnm3 locus. Copyright 2005 [PMID:15885504]

Expression pattern clarity: three stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:5010Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
5010_wholemount_moderate_3D_1.wlz
5010_wholemount_weak_3D_1.wlz
5010_wholemount_notDetected_3D_1.wlz
5010_wholemount_strong_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:5010_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: three stars
Text Annotation:
StructureLevelPatternNotes
1st branchial arch
detected detected
2nd branchial arch
detected detected
forelimb bud
detected detected
hindlimb bud
detected detected
trunk somite
detected detected
nasal process
detected detected
branchial arch
detected detected
regionalExpression is detected in the nasal, ventral periorbital, facial, maxillary and cervical tissues.
nose
detected detected
regionalExpression is detected in the nasal, ventral periorbital, facial, maxillary and cervical tissues.
axial skeleton cervical region
detected detected
regionalExpression is detected in the nasal, ventral periorbital, facial, maxillary and cervical tissues.
perioptic mesenchyme
detected detected
regionalExpression is detected in the nasal, ventral periorbital, facial, maxillary and cervical tissues.
maxillary process
detected detected
regionalExpression is detected in the nasal, ventral periorbital, facial, maxillary and cervical tissues.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:3587750
Entity Detected:Dnm3os, dynamin 3, opposite strand ( MGI:3052332)
Sequence:sense strand is shown

>MGI:3587750
TGTGAGAGTTGAAAAATCTTAATTATTTTTAAAAAAAAGAGTTGAGATTATATATATATCCACACATACA
TATCCCTTTGTTTGCACAGGAAATTAAAAGCACTATTAGTTCACTTATGGAAGTTGTGTGTCTCAGGAAA
CACACTTTCTTGACTAACGCGTCACGAAAGAGGACAGAGAAGAATCACTTGGATCATATTTAAGATTTCT
CTAATTTTGAGGGCCTATTGTATACTAGAAATAATTGAATTTTATAACTTGCCTTTATTTAATGATATTT
AATTTAAAATTTTCTCTTCATATTAAATGCATTTTTTTTAATTTCAAGAAGAATAACCTTTCTTCACAGG
TCCCTTCCCTATTTCTCCCATGGGACAGTTTTTAGTCTGAAGATACAAAGGGCATAAGGGTGATTCTCCT
GTAAGTTCAACAGTAAAAAGTAACAATTTCTTTGTAAACCCGGAGTCTCTGCCTGTTCTTGGTTGGAGTT
CTAACACACCATTTTCAAGGAAAATTCTAAATGAATGAAGGAGTGTGGTACTTAATTTTAAATGTATGAG
TTATATTAAAAGTACAAAAAAGTTGTTTGATAACCCTCTGCTGTATGAAGACATGGTGTTAAGCCTGTGT
GCTGGTGGATATATCCCTTATAGACACTTTAGGATAAAAATAAAGATATCGATTCC
Notes:The Dnm3os probe used in this study by Loebel et al., 2005 [PMID:15885504] is described as follows. "Digoxigenin-labeled probes were prepared using Ampliscribe (Epicentre) from linearized DNA of clone M24 (Loebel et al., 2002 [PMID:12124942] )." Editors Note: BB043240 is a sequence identifier for clone M24 given in Table 1 of Loebel et al., 2002 [PMID:12124942] .
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:ARC/S
Age:11.5 dpc
Theiler Stage:TS19
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Fixation:4% paraformaldehyde
Staining procedure:alkaline phosphatase + unspecified
General Information
Authors:Loebel DA; Tsoi B; Wong N; Tam PP, 2005 [PMID:15885504] , Indexed by GXD, Spatially Mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1016/j.ygeno.2005.02.001] [ PMID:15885504] Loebel DA, Tsoi B, Wong N, Tam PP 2005 A conserved noncoding intronic transcript at the mouse Dnm3 locus. Genomics (85):782-9
 [ doi:10.1002/gene.10091] [ PMID:12124942] Loebel DA, O'Rourke MP, Steiner KA, Banyer J, Tam PP 2002 Isolation of differentially expressed genes from wild-type and Twist mutant mouse limb buds. Genesis (33):103-13
Links:MGI:3587779 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI