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EMAGE:5014

Dscam Down syndrome cell adhesion molecule ( MGI:1196281)
TS18 (10 dpc)
in situ hybridisation

Data Images
EMAGE:5014 EMAGE:5014
Fig4A. [doi:10.1006/bbrc.2001.4420] Reprinted from Biochem Biophys Res Comm, vol 281, Agarwala KL; Ganesh S; Amano K; Suzuki T; Yamakawa K; "Dscam, a highly conserved gene in mammals, expressed in differentiating mouse brain", pp 697-705, Copyright (2001) with permission from Elsevier. [PMID:11237714] Fig4D. [doi:10.1006/bbrc.2001.4420] Reprinted from Biochem Biophys Res Comm, vol 281, Agarwala KL; Ganesh S; Amano K; Suzuki T; Yamakawa K; "Dscam, a highly conserved gene in mammals, expressed in differentiating mouse brain", pp 697-705, Copyright (2001) with permission from Elsevier. [PMID:11237714]

Expression pattern clarity: three stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
Image annotations: T, telencephalon; D, diencephalon; M, mid brain; Cb, cerebellar plate; Rh, rhombencephalon; H, hind brain. Fig4a is a lateral view, Fig4d is a rostral view. Editors note: the authors define the strain used as "C57 BALB/c".
Expression Pattern Description
Spatial Annotation:
EMAGE:5014Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
5014_wholemount_moderate_3D_1.wlz
5014_wholemount_possible_3D_1.wlz
5014_wholemount_notDetected_3D_1.wlz
5014_wholemount_strong_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:5014_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Text Annotation:
StructureLevelPatternNotes
hindbrain
detected detected
regionalexpression was specifically observed in the hindbrain (cerebellar plate neurons), midbrain and forebrain.
forebrain
detected detected
expression was specifically observed in the hindbrain (cerebellar plate neurons), midbrain and forebrain.
midbrain
detected detected
expression was specifically observed in the hindbrain (cerebellar plate neurons), midbrain and forebrain.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:Dscam probeA
Entity Detected:Dscam, Down syndrome cell adhesion molecule ( MGI:1196281)
Notes:The Dscam probe used in this study by Agarwala et al., 2001 [PMID:11237714] was transcribed from "1.5 kb of the mouse cDNA clone corresponding to the 5' coding region, subcloned into Bluescript vector SK1/2 (Stratagene)."
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6J x BALB/c
Age:10 dpc
Theiler Stage:TS18
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Fixation:4% paraformaldehyde
Staining procedure:alkaline phosphatase + BMpurple
General Information
Authors:Agarwala KL; Ganesh S; Amano K; Suzuki T; Yamakawa K, 2001 [PMID:11237714] , Indexed and Spatially Mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1006/bbrc.2001.4420] [ PMID:11237714] Agarwala KL, Ganesh S, Amano K, Suzuki T, Yamakawa K 2001 DSCAM, a highly conserved gene in mammals, expressed in differentiating mouse brain. Biochem Biophys Res Commun (281):697-705
Links: Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEMAGE