EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:5015

Alx4 aristaless-like homeobox 4 ( MGI:108359)
TS19 (11.5 dpc)
in situ hybridisation

Data Images


Raw signal output, as movie.	All sections along the X-axis, as movie.	All sections along the Y-axis, as movie.	All sections along the Z-axis, as movie.	Photograph prior to 3D imaging.

Photograph prior to 3D imaging.	Photograph prior to 3D imaging. Alx4 expression in medial and lateral nasal processes, in naso-lacrimal groove and in groove between nasal processes and telencephalic vesicle.	Sample morphology following 3D imaging, as movie

View 3D opt image:
Download 3D images in woolz format
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Notes:

This is a traditionally produced wholemount in situ hybridisation stained using alkaline phosphatase + BM Purple. It has been imaged using Optical Projection Tomography. The visible channel depicting the signal has been false-coloured yellow and the fluorescence channel depicting autofluorescence has been false-coloured blue.

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

5015_wholemount_moderate_3D_1.wlz

5015_wholemount_possible_3D_1.wlz

5015_wholemount_notDetected_3D_1.wlz

5015_wholemount_strong_3D_1.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:5015_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

latero-nasal process

strong

medial-nasal process

strong

naso-lacrimal groove

moderate

Annotation Validation:

Submitter + EMAGE Editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	Alx4_cloneB
Entity Detected:	Alx4, aristaless-like homeobox 4 ( MGI:108359)
Sequence:	sense strand is shown >Alx4_cloneB GGACTGCTGGGAGGGAGGAGGCGATGGTAGGCTCTTGTCCCTCCCTGGCTCTGAGGTCATGACCCCTGCT CCCTGCCAAAGGCAGTGGAGAGAGGCAGGCGGCTCTGTGCGTACCTTTGCATCATGTAAGCTGAGACCCT GTCTTTCTTCGTGTTCTTCCACCCCTTCCGTTTGACTTGAGTCAAAGTCAGGCCCCCTTCCAGATCTACC AGCTTTAACAGTTCAGCCTTGCCTAACCTTCCTTTCTCCTGTGGGGTATCCTGGTCGCTGGGCCTGTGGC TGTGTGTCTTAGAACCAAGCTTCCGTGCAGGGTCAGCTCCCGATCCTCTTTGCTCCTTGAAAACATAGAA CCCCACTTTCCGTATCGAAGGCATCTGGTCATCCACTCATCCCATGAGCCCTGTGACTGTAGCTTCACTG TCCCCTGTGATGGATGTGCCTGGAAGATGTGGGAGATGAGAGGAGGGGTCAGAGCTGAAAGGACTCTGCT GCTGTCCACCCATAAGCCCCATGGGGACGTGGGCCAGCCTTTGCTCCTGCAGGTGGTATTGGTTGACACT GTGAAGGTGATGTGCCAGGTGTGAAGGGAGGCAAGAACATAGGTTTTTTTTTTTTTTTCTCCCATTATTA CCACCACACATGGACACTGGGAGCCAGAGAAGCTCTCAGAATGATGGCCATGGGGATGATCCAGCGGGCT GAACCTGGTGTCTTCTGTGGCGGAGAGGTCCTTTGGTCTGCACTGGCGCCTCCTCAGGGCACCTCTGGGA GGAGTTGAGATATTTATCCTCTCCCTCCTGGTCACTCACTGGGGCTGGCTGGGCTGGAAGAATTC
	nt 4302 - nt 3468 of BC049786.1
Notes:	This Alx4 probe template fragment (600bp), cloned in pBluescript, was kindly donated by Paul Sharpe. To generate Alx4 antisense probe, the plasmid was cut with EcoRV and transcribed from the T7 promoter. Probe available on request.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	CD-1
Age:	11.5 dpc
Theiler Stage:	TS19
Mutations:	none (wild-type)
Preparation:	opt

Procedures

Fixation:	4% paraformaldehyde
Embedding:	agarose
Staining procedure:	alkaline phosphatase + BMpurple

General Information

Authors:	Laura Ward, Mike Dixon
Principal investigator:	Mike Dixon, University of Manchester, Faculty of Life Sciences Michael Smith Building Oxford Road, Manchester, England M13 9PT
Submitted by:	Laura Ward, University of Manchester, Faculty of Life Sciences Michael Smith Building Oxford Road, Manchester, England M13 9PT
Experiment type:	screen
Links:	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source