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EMAGE:5018

Alx4 aristaless-like homeobox 4 ( MGI:108359)
TS22 (12.5 dpc)
in situ hybridisation

Data Images
EMAGE:5018 EMAGE:5018 EMAGE:5018 EMAGE:5018 EMAGE:5018
Raw signal output, as movie. All sections along the X-axis, as movie. All sections along the Y-axis, as movie. All sections along the Z-axis, as movie. Photograph prior to 3D imaging.
EMAGE:5018 EMAGE:5018
Photograph prior to 3D imaging. Alx4 expression in leading edge of lip. No expression detected in primary or secondary palate. Sample morphology following 3D imaging, as movie

View 3D opt image: EMAGE genex expression entry
Download 3D images in woolz format
Expression pattern clarity: three stars
Notes:
This is a traditionally produced wholemount in situ hybridisation stained using alkaline phosphatase + BM Purple. It has been imaged using Optical Projection Tomography. The visible channel depicting the signal has been false-coloured yellow and the fluorescence channel depicting autofluorescence has been false-coloured blue.
Expression Pattern Description
Text Annotation:
StructureLevelPatternNotes
palatal shelf epithelium
not detected not detected
homogeneous
palatal shelf mesenchyme
not detected not detected
homogeneous
rest of upper lip
moderate moderate
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:Alx4_cloneB
Entity Detected:Alx4, aristaless-like homeobox 4 ( MGI:108359)
Sequence:sense strand is shown

>Alx4_cloneB
GGACTGCTGGGAGGGAGGAGGCGATGGTAGGCTCTTGTCCCTCCCTGGCTCTGAGGTCATGACCCCTGCT
CCCTGCCAAAGGCAGTGGAGAGAGGCAGGCGGCTCTGTGCGTACCTTTGCATCATGTAAGCTGAGACCCT
GTCTTTCTTCGTGTTCTTCCACCCCTTCCGTTTGACTTGAGTCAAAGTCAGGCCCCCTTCCAGATCTACC
AGCTTTAACAGTTCAGCCTTGCCTAACCTTCCTTTCTCCTGTGGGGTATCCTGGTCGCTGGGCCTGTGGC
TGTGTGTCTTAGAACCAAGCTTCCGTGCAGGGTCAGCTCCCGATCCTCTTTGCTCCTTGAAAACATAGAA
CCCCACTTTCCGTATCGAAGGCATCTGGTCATCCACTCATCCCATGAGCCCTGTGACTGTAGCTTCACTG
TCCCCTGTGATGGATGTGCCTGGAAGATGTGGGAGATGAGAGGAGGGGTCAGAGCTGAAAGGACTCTGCT
GCTGTCCACCCATAAGCCCCATGGGGACGTGGGCCAGCCTTTGCTCCTGCAGGTGGTATTGGTTGACACT
GTGAAGGTGATGTGCCAGGTGTGAAGGGAGGCAAGAACATAGGTTTTTTTTTTTTTTTCTCCCATTATTA
CCACCACACATGGACACTGGGAGCCAGAGAAGCTCTCAGAATGATGGCCATGGGGATGATCCAGCGGGCT
GAACCTGGTGTCTTCTGTGGCGGAGAGGTCCTTTGGTCTGCACTGGCGCCTCCTCAGGGCACCTCTGGGA
GGAGTTGAGATATTTATCCTCTCCCTCCTGGTCACTCACTGGGGCTGGCTGGGCTGGAAGAATTC
nt 4302 - nt 3468 of BC049786.1
Notes:This Alx4 probe template fragment (600bp), cloned in pBluescript, was kindly donated by Paul Sharpe. To generate Alx4 antisense probe, the plasmid was cut with EcoRV and transcribed from the T7 promoter. Probe available on request.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:CD-1
Age:12.5 dpc
Theiler Stage:TS22
Mutations:none (wild-type)
Preparation:opt
Procedures
Fixation:4% paraformaldehyde
Embedding:agarose
Staining procedure:alkaline phosphatase + BMpurple
General Information
Authors:Laura Ward, Mike Dixon
Principal investigator:Mike Dixon, University of Manchester, Faculty of Life Sciences Michael Smith Building Oxford Road, Manchester, England M13 9PT
Submitted by:Laura Ward, University of Manchester, Faculty of Life Sciences Michael Smith Building Oxford Road, Manchester, England M13 9PT
Experiment type:screen
Links: Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceFACEBASE