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EMAGE:5027

Scube1 signal peptide, CUB domain, EGF-like 1 ( MGI:1890616)
TS15 (9.5 dpc)
in situ hybridisation

Data Images
EMAGE:5027
Figure 3d. [doi:10.1006/geno.2000.6370] Grimmond S; Larder R; Van Hateren N; Siggers P; Hulsebos TJ; Arkell R; Greenfield A, "Cloning, mapping, and expression analysis of a gene encoding a novel mammalian EGF-related protein (SCUBE1)" Genomics 2000 Nov 15;70(1):74-81. [PMID:11087664]

Expression pattern clarity: three stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:5027Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
5027_wholemount_moderate.wlz
5027_wholemount_weak.wlz
5027_wholemount_possible.wlz
5027_wholemount_notDetected.wlz
5027_wholemount_strong.wlz
(what is wlz format?)
Download all expression domains: EMAGE:5027_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
trunk somite
detected detected
regionalExpression is present in the dorsal-most region of the somites.
surface ectoderm
detected detected
Expression is seen throughout the surface ectoderm.
infundibular recess of 3rd ventricle
detected detected
Expression is present in the infundibulum
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:3710133
Entity Detected:Scube1, signal peptide, CUB domain, EGF-like 1 ( MGI:1890616)
Notes:The Scube1 probe used in this study by Grimmond et al., 2000 [PMID:11087664] was generated from "the NMUR clone 1C" following linearisation with EcoRI. See Figure 1A therein for a cartoon representation of the region covered by this clone.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:(C3H x 101)F1
Age:9.5 dpc
Theiler Stage:TS15
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Fixation:4% paraformaldehyde
General Information
Authors:Grimmond S; Larder R; Van Hateren N; Siggers P; Hulsebos TJ; Arkell R; Greenfield A, 2000 [PMID:11087664] . Indexed by GXD, Spatially mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1006/geno.2000.6370] [ PMID:11087664] Grimmond S, Larder R, Van Hateren N, Siggers P, Hulsebos TJ, Arkell R, Greenfield A 2000 Cloning, mapping, and expression analysis of a gene encoding a novel mammalian EGF-related protein (SCUBE1). Genomics (70):74-81
Links:MGI:3710143 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI