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EMAGE:5034

Sema6c sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6C ( MGI:1338032)
TS19 (13 dpc)
in situ hybridisation

Data Images
EMAGE:5034
Figure 4B 6-CLP. [doi:10.1074/jbc.M206451200] Qu X; Wei H; Zhai Y; Que H; Chen Q; Tang F; Wu Y; Xing G; Zhu Y; Liu S; Fan M; He F, "Identification, characterization, and functional study of the two novel human members of the semaphorin gene family." J Biol Chem 2002 Sep 20;277(38):35574-85. [PMID:12110693]

Expression pattern clarity: three stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:5034Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
5034_wholemount_moderate.wlz
5034_wholemount_weak.wlz
5034_wholemount_possible.wlz
5034_wholemount_notDetected.wlz
5034_wholemount_strong.wlz
(what is wlz format?)
Download all expression domains: EMAGE:5034_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Text Annotation:
StructureLevelPatternNotes
lateral ventricle
detected detected
brain
detected detected
Expressed in striatum
midbrain lateral wall
detected detected
midbrain
detected detected
regionalExpressed in pons/midbrain junction
hindbrain
detected detected
regionalExpressed in pons/midbrain junction
future spinal cord
detected detected
Annotation Validation: Submitter + EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:Sema6c probeA
Entity Detected:Sema6c, sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6C ( MGI:1338032)
Notes:The Sema6c probe used in this study by Qu et al., 2002 [PMID:12110693] was "6-CLP", "SEMA6C long isoform probe". Editors Note: The sequence of this probe is not described by the authors, however elsewhere in the experimental procedures section, the authors do report that the following primers were used to detect the presence of a 120-bp insertion in SEMA6C (as opposed to a 96-bp insertion) in RT-PCR experiments: P9, 5'-GTCTGCGCCTTCTACCTGGA-3'; and P10, 5'-GGCCTGTAAAACATCAAAT-3'. These may have been used to generate the probe template sequence.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Age:13 dpc
Theiler Stage:TS19
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Fixation:4% paraformaldehyde
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Qu X; Wei H; Zhai Y; Que H; Chen Q; Tang F; Wu Y; Xing G; Zhu Y; Liu S; Fan M; He F, 2002 [PMID:12110693] . Indexed and spatially mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1074/jbc.M206451200] [ PMID:12110693] Qu X, Wei H, Zhai Y, Que H, Chen Q, Tang F, Wu Y, Xing G, Zhu Y, Liu S, Fan M, He F 2002 Identification, characterization, and functional study of the two novel human members of the semaphorin gene family. J Biol Chem (277):35574-85
Links: Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEMAGE