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EMAGE:5037

Sf3b1 splicing factor 3b, subunit 1 ( MGI:1932339)
TS18 (10.5 dpc)
in situ hybridisation

Data Images
EMAGE:5037
Figure 5C. [doi:10.1007/s003350010258] Isono K; Abe K; Tomaru Y; Okazaki Y; Hayashizaki Y; Koseki H, "Molecular cloning, genetic mapping, and expression of the mouse Sf3b1 (SAP155) gene for the U2 snRNP component of spliceosome." Mamm Genome 2001 Mar;12(3):192-8. [PMID:11252167]

Expression pattern clarity: two stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:5037Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
5037_wholemount_moderate_3D_1.wlz
5037_wholemount_possible_3D_1.wlz
5037_wholemount_strong_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:5037_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
limb
strong strong
Expression is stronger in limb buds than in other tissues.
embryo
detected detected
Nearly ubiquitous expression.
midbrain
strong strong
regionalExpression in dorsal region.
hindbrain
strong strong
regionalExpression in dorsal region.
neural tube
strong strong
gradedThere is a dorsoventral gradient in the anterior neural tube.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:Sf3b1 probeA
Entity Detected:Sf3b1, splicing factor 3b, subunit 1 ( MGI:1932339)
Sequence:sense strand is shown

>Sf3b1 probeA
GTTCCGTCTGTGTGTTCGAGTGGACAAAATGGCGAAGATCGCCAAGACTCACGAAGATATCGAAGCACAG
ATTCGAGAAATTCAAGGCAAGAAGGCAGCTCTTGATGAAGCCCAAGGAGTGGGCCTTGATTCCACAGGTT
ATTATGACCAAGAAATTTATGGTGGAAGTGATAGCAGATTTGCTGGATATGTGACATCAATTGCTGCAAC
TGAACTTGAAGATGATGACGATGACTACTCATCATCCACGAGCTTGCTCGGTCAGAAGAAGCCTGGATAT
CATGCCCCCGTGGCGTTGCTTAATGATATACCACAGTCAACAGAGCAGTATGATCCATTTGCTGAGCATC
GTCCTCCAAAGATTGCCGATCGGGAAGATGAATACAAAAAGCATAGGCGGACCATGATAATTTCCCCAGA
GCGTCTTGATCCTTTTGCAGATGGAGGGAAGACCCCCGATCCTAAAATGAATGCTAGAACCTACATGGAT
GTTATGCGAGAACAACATTTGACTAAGGAAGAGAGAGAAATTAGGCAACAACTAGCAGAAAAAGCTAAAG
CTGGAGAACTAAAAGTCGTCAATGGAGCAGCAGCATCCCAGCCTCCCTCAAAACGAAAACGGCGCTGGGA
TCAGACCGCTGACCAGACTCCTGGTGCCACTCCCAAAAAGCTATCGAGTTGGGATCAGGCAGAGACCCCT
GGGCATACCCCATCTTTAAGATGGGATGAGACACCGGGTCGTGCAAAAGGAAGTGAAACACCTGGCGCAA
CCCCAGGCTCAAAAATATGGGACCCTACACCGAGTCATACACCTGCGGGAGCTGCTACTCCTGGGCGAGG
CGACACACCAGGCCATGCAACCCCGGGCCATGGTGGTGCAACTTCCAGTGCTCGTAAAAACAGATGGGAT
GAGACCCCCAAAACAGAAAGAGATACTCCTGGGCATGGAAGTGGATGGGCTGAGACTCCTCGAACAGATC
GAGGTGGAGACTCTATTGGTGAAACACCAACTCCTGGAGCAAGTAAAAGAAAGTCTCGTTGGGATGAAAC
GCCGGCGAGTCAAATGGGTGGAAGCACTCCTGTTCTGACTCCAGGAAAAACACCAATTGGCACACCAGCC
ATGAACATGGCTACCCCTACTCCAGGTCACATAATGAGCATGACACCTGAACAGCTTCAGGCGTGGCGGT
GGGAGAGAGAAATTGATGAACGAAACCGCCCACTTTCTGATGAAGAATTAGATGCTATGTTCCCAGAAGG
ATATAAGGTACTTCCCCCTCCAGCTGGTTATGTTCCTATTCGAACTCCAGCTCGAAAGCTGACAGCAACT
CCTACACCTTTGGGTGGTATGACTGGTTTCCACATGCAAACTGAAGACAGAACTATGAAAAGTGTCAATG
ACCAGCCATCTGGGAATCTTCCATTTTTAAAACCTGATGACATTCAGTACTTTGATAAACTATTGGTTGA
TGTAGATGAATCAACACTTAGTCCAGAAGAACAAAAAGAAAGAAAAATAATGAAGTTGCTTTTAAAAATT
AAGAATGGTACACCTCCAATGAGAAAGGCTGCCTTACGTCAGATTACTGATAAAGC
nt 110 - nt 1705 of AB037890.1
Notes:The Sf3b1 probe used in this study by Isono et al., 2001 [PMID:11252167] is described as follows "Digoxigenin (DIG)-labeled antisense RNA was synthesized by T7 RNA polymerase from pSAP-5'Xh linearized by EcoRI digestion". Construction of pSAP-5'Xh was achieved by amplifying a fragment by RT-PCR using the following primers: 5'-primer, 5'GTTCCGTCTGTGTGTTCGAG3', and a 3'-primer, 5'TTGGGCCCATCCCTCCAGTATAGTG3'. The PCR product was cut with XhoI and the 5' end cloned into pBluescript SK to make pSAP-5'Xh. Editor's note: AB037890.1 is the cDNA sequence deposited in GenBank by the authors. The 5' primer extends between nt 110-129 of AB037890.1 and there is one XhoI site present in AB037890.1 at nt 1705. No EcoRI sites are found within this region therefore pSAP-5'Xh was linearised in the vector.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Age:10.5 dpc
Theiler Stage:TS18
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Isono K; Abe K; Tomaru Y; Okazaki Y; Hayashizaki Y; Koseki H, 2001 [PMID:11252167] . Indexed and spatially mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1007/s003350010258] [ PMID:11252167] Isono K, Abe K, Tomaru Y, Okazaki Y, Hayashizaki Y, Koseki H 2001 Molecular cloning, genetic mapping, and expression of the mouse Sf3b1 (SAP155) gene for the U2 snRNP component of spliceosome. Mamm Genome (12):192-8
Links: Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEMAGE