Type: | in situ hybridisation probe |
Identifier: | Sf3b1 probeA |
Entity Detected: | Sf3b1, splicing factor 3b, subunit 1 ( MGI:1932339) |
Sequence: | sense strand is shown
>Sf3b1 probeA
GTTCCGTCTGTGTGTTCGAGTGGACAAAATGGCGAAGATCGCCAAGACTCACGAAGATATCGAAGCACAG
ATTCGAGAAATTCAAGGCAAGAAGGCAGCTCTTGATGAAGCCCAAGGAGTGGGCCTTGATTCCACAGGTT
ATTATGACCAAGAAATTTATGGTGGAAGTGATAGCAGATTTGCTGGATATGTGACATCAATTGCTGCAAC
TGAACTTGAAGATGATGACGATGACTACTCATCATCCACGAGCTTGCTCGGTCAGAAGAAGCCTGGATAT
CATGCCCCCGTGGCGTTGCTTAATGATATACCACAGTCAACAGAGCAGTATGATCCATTTGCTGAGCATC
GTCCTCCAAAGATTGCCGATCGGGAAGATGAATACAAAAAGCATAGGCGGACCATGATAATTTCCCCAGA
GCGTCTTGATCCTTTTGCAGATGGAGGGAAGACCCCCGATCCTAAAATGAATGCTAGAACCTACATGGAT
GTTATGCGAGAACAACATTTGACTAAGGAAGAGAGAGAAATTAGGCAACAACTAGCAGAAAAAGCTAAAG
CTGGAGAACTAAAAGTCGTCAATGGAGCAGCAGCATCCCAGCCTCCCTCAAAACGAAAACGGCGCTGGGA
TCAGACCGCTGACCAGACTCCTGGTGCCACTCCCAAAAAGCTATCGAGTTGGGATCAGGCAGAGACCCCT
GGGCATACCCCATCTTTAAGATGGGATGAGACACCGGGTCGTGCAAAAGGAAGTGAAACACCTGGCGCAA
CCCCAGGCTCAAAAATATGGGACCCTACACCGAGTCATACACCTGCGGGAGCTGCTACTCCTGGGCGAGG
CGACACACCAGGCCATGCAACCCCGGGCCATGGTGGTGCAACTTCCAGTGCTCGTAAAAACAGATGGGAT
GAGACCCCCAAAACAGAAAGAGATACTCCTGGGCATGGAAGTGGATGGGCTGAGACTCCTCGAACAGATC
GAGGTGGAGACTCTATTGGTGAAACACCAACTCCTGGAGCAAGTAAAAGAAAGTCTCGTTGGGATGAAAC
GCCGGCGAGTCAAATGGGTGGAAGCACTCCTGTTCTGACTCCAGGAAAAACACCAATTGGCACACCAGCC
ATGAACATGGCTACCCCTACTCCAGGTCACATAATGAGCATGACACCTGAACAGCTTCAGGCGTGGCGGT
GGGAGAGAGAAATTGATGAACGAAACCGCCCACTTTCTGATGAAGAATTAGATGCTATGTTCCCAGAAGG
ATATAAGGTACTTCCCCCTCCAGCTGGTTATGTTCCTATTCGAACTCCAGCTCGAAAGCTGACAGCAACT
CCTACACCTTTGGGTGGTATGACTGGTTTCCACATGCAAACTGAAGACAGAACTATGAAAAGTGTCAATG
ACCAGCCATCTGGGAATCTTCCATTTTTAAAACCTGATGACATTCAGTACTTTGATAAACTATTGGTTGA
TGTAGATGAATCAACACTTAGTCCAGAAGAACAAAAAGAAAGAAAAATAATGAAGTTGCTTTTAAAAATT
AAGAATGGTACACCTCCAATGAGAAAGGCTGCCTTACGTCAGATTACTGATAAAGC
|
| nt 110 - nt 1705 of AB037890.1 |
Notes: | The Sf3b1 probe used in this study by Isono et al., 2001 [PMID:11252167] is described as follows "Digoxigenin (DIG)-labeled antisense RNA was synthesized by T7 RNA polymerase from pSAP-5'Xh linearized by EcoRI digestion". Construction of pSAP-5'Xh was achieved by amplifying a fragment by RT-PCR using the following primers: 5'-primer, 5'GTTCCGTCTGTGTGTTCGAG3', and a 3'-primer, 5'TTGGGCCCATCCCTCCAGTATAGTG3'. The PCR product was cut with XhoI and the 5' end cloned into pBluescript SK to make pSAP-5'Xh.
Editor's note: AB037890.1 is the cDNA sequence deposited in GenBank by the authors. The 5' primer extends between nt 110-129 of AB037890.1 and there is one XhoI site present in AB037890.1 at nt 1705. No EcoRI sites are found within this region therefore pSAP-5'Xh was linearised in the vector. |
Chemistry: | RNA |
Strand: | antisense |
Label: | digoxigenin |