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EMAGE:5038

Sfrp1 secreted frizzled-related protein 1 ( MGI:892014)
TS15 (9.5 dpc)
in situ hybridisation

Data Images
EMAGE:5038
Figure 5E. Copyright: This image is from [doi:10.1002/(SICI)1097-0177(199807)212:3<364::AID-AJA4>3.0.CO;2-F] Hoang BH; Thomas JT; Abdul-Karim FW; Correia KM; Conlon RA; Luyten FP; Ballock RT, "Expression pattern of two Frizzled-related genes, Frzb-1 and Sfrp-1, during mouse embryogenesis suggests a role for modulating action of Wnt family members." Dev Dyn 1998, 212:364-72. Reprinted with permission of Wiley-Liss Inc. [PMID:9671940]

Expression pattern clarity: three stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
Image annotations: pe, posterior optic eminence and eye; vhb, ventral hindbrain.
Expression Pattern Description
Spatial Annotation:
EMAGE:5038Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
5038_wholemount_possible_3D_1.wlz
5038_wholemount_notDetected_3D_1.wlz
5038_wholemount_strong_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:5038_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: three stars
Text Annotation:
StructureLevelPatternNotes
eye
detected detected
regionalExpressed in posterior optic eminence.
future hindbrain
detected detected
regionalExpressed in ventral hindbrain with the anterior most limit at the isthmus.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:Sfrp1 probeA
Entity Detected:Sfrp1, secreted frizzled-related protein 1 ( MGI:892014)
Sequence:sense strand is shown

>Sfrp1 probeA
CGCTTCTACACCAAGCCCCCGCAGTGCGTGGACATCCCGGTGGACCTGAGGCTGTGCCACAACGTGGGCT
ACAAGAAGATGGTGCTGCCCAACCTGCTGGAGCACGAGACCATGGCAGAGGTGAAGCAGCAGGCCAGCAG
CTGGGTGCCGCTGCTCAACAAGAACTGCCACATGGGCACCCAGGTCTTCCTCTGTTCGCTCTTCGCGCCC
GTCTGTCTGGACCGGCCCATCTACCCGTGTCGCTGGCTCTGCGAGGCCGTGCGCGACTCGTGCGAGCCGG
TCATGCAGTTCTTCGGCTTCTACTGGCCCGAGATGCTCAAATGTGACAAGTTCCCCGAGGGCGACGTCTG
CATCGCCATGACCCCGCCCAATACCACGGAAGCCTCTAAGCCCCAAGGTACAACCGTGTGTCCTCCATGC
GACAACGAGTTGAAGTCAGAGGCCATCATTGAACATCTCTGTGCAAGCGAGTTTGCACTGAGGATGAAAA
TCAAAGAAGTGAAGAAGGAAAACGGTGACAAGAAGATTGTCCCCAAGAAGAAGAAACCCTTGAAGCTGGG
GCCCATCAAGAAGAAGGAGCTGAAGG
nt 404 - nt 989 of NM_013834.1
Notes:The Sfrp1 probe template used in this study by Hoang et al., 1998 [PMID:9671940] is indicated as that originally described by Rattner et al., 1997 [PMID:9096311] which "contained a 585-bp insert from codon 49 to codon 244".
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:CD-1
Age:9.5 dpc
Theiler Stage:TS15
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Fixation:4% paraformaldehyde
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Hoang BH; Thomas JT; Abdul-Karim FW; Correia KM; Conlon RA; Luyten FP; Ballock RT, 1998 [PMID:9671940] . Indexed and spatially mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1002/(SICI)1097-0177(199807)212:3<364::AID-AJA4>3.0.CO;2-F] [ PMID:9671940] Hoang BH, Thomas JT, Abdul-Karim FW, Correia KM, Conlon RA, Luyten FP, Ballock RT 1998 Expression pattern of two Frizzled-related genes, Frzb-1 and Sfrp-1, during mouse embryogenesis suggests a role for modulating action of Wnt family members. Dev Dyn (212):364-72
 [ doi:10.1073/pnas.94.7.2859] [ PMID:9096311] Rattner A, Hsieh JC, Smallwood PM, Gilbert DJ, Copeland NG, Jenkins NA, Nathans J 1997 A family of secreted proteins contains homology to the cysteine-rich ligand-binding domain of frizzled receptors. Proc Natl Acad Sci U S A (94):2859-63
Links: Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEMAGE