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EMAGE:5039

Sfrp2 secreted frizzled-related protein 2 ( MGI:108078)
TS16 (10.5 dpc)
in situ hybridisation

Data Images
EMAGE:5039
Figure 1D. Copyright: This image is from [doi:10.1002/(SICI)1097-0177(199812)213:4<440::AID-AJA9>3.0.CO;2-6] Lescher B; Haenig B; Kispert A, "sFRP-2 is a target of the Wnt-4 signaling pathway in the developing metanephric kidney." Dev Dyn 1998 Dec;213(4):440-51. Reprinted with permission of Wiley-Liss Inc. [PMID:9853965]

Expression pattern clarity: three stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
Note: ba, branchial arch; fli, forelimb bud; mt, mesonephric tubule; spc, spinal cord.
Expression Pattern Description
Spatial Annotation:
EMAGE:5039Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
5039_wholemount_moderate_3D_1.wlz
5039_wholemount_weak_3D_1.wlz
5039_wholemount_possible_3D_1.wlz
5039_wholemount_notDetected_3D_1.wlz
5039_wholemount_strong_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:5039_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Text Annotation:
StructureLevelPatternNotes
future midbrain
detected detected
regionalExpression is resolved into two horizontal stripes.
future spinal cord
detected detected
regionalExpression is beginning to retract from the lateral regions to a thin band of cells located on each side of the lumen possibly representing ependymal cells.
fronto-nasal process
detected detected
forelimb bud
weak weak
Weak expression in the hand plate of the forelimb buds.
1st branchial arch mandibular component
weak weak
2nd branchial arch
weak weak
trunk somite
weak weak
regionalExpressed weakly in the posterior half of the somites.
eye
detected detected
future hindbrain
detected detected
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:Sfrp2 probeB
Entity Detected:Sfrp2, secreted frizzled-related protein 2 ( MGI:108078)
Sequence:sense strand is shown

>Sfrp2 probeB
TTCGGCACAGGCCTCGTCACTCGCTCTCGTGCAGTGAAGGACCGCTGCGCCCGGTCATGTCCGCCTTCGC
TTCCCCTGGCCAGACATGCTGGAGTGCGACCGTTTCCCGCAGACAACGACCTCTGCATCCCCCTCGCTAG
TAGCGACCACCTCCTGCCGGCCACAGAGGAAGCTCCCAAGGTGTGTGAAGCCTGCAAAACCAAGAATGAG
GACGACAACGACATCATGGAAACCCTTTGTAAAAATGACTTCGCACTGAAAATCAAAGTGAAGGAGATAA
CGTACATCAACAGAGACAC
Notes:The Sfrp2 probe used in this study by Lescher et al. 1998 [PMID:9853965] is described as follows: "A probe corresponding to approximately 1 kb of the sFRP-2 full-length cDNA was generated from the plasmid pT7T3.fz10(1.0) (Genome System EST clone W08345) by linearisation with EcoRI and transcription with T3 RNA polymerase".
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:NMRI
Age:10.5 dpc
Theiler Stage:TS16
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Fixation:4% paraformaldehyde
Staining procedure:alkaline phosphatase + BMpurple
General Information
Authors:Lescher B; Haenig B; Kispert A, 1998 [PMID:9853965] . Indexed and spatially mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1002/(SICI)1097-0177(199812)213:4<440::AID-AJA9>3.0.CO;2-6] [ PMID:9853965] Lescher B, Haenig B, Kispert A 1998 sFRP-2 is a target of the Wnt-4 signaling pathway in the developing metanephric kidney. Dev Dyn (213):440-51
Links: Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEMAGE