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EMAGE:5040

Sfrp2 secreted frizzled-related protein 2 ( MGI:108078)
TS15 (9.5 dpc)
in situ hybridisation

Data Images
EMAGE:5040
Figure 1C. Copyright: This image is from [doi:10.1002/(SICI)1097-0177(199812)213:4<440::AID-AJA9>3.0.CO;2-6] Lescher B; Haenig B; Kispert A, "sFRP-2 is a target of the Wnt-4 signaling pathway in the developing metanephric kidney." Dev Dyn 1998 Dec;213(4):440-51. Reprinted with permission of Wiley-Liss Inc. [PMID:9853965]

Expression pattern clarity: three stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
Image annotations: hb - hindbrain; mb - midbrain; mm - mesonephric mesenchyme; ov - optic vesicle; spc - spinal cord.
Expression Pattern Description
Spatial Annotation:
EMAGE:5040Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
5040_wholemount_moderate.wlz
5040_wholemount_weak.wlz
5040_wholemount_notDetected.wlz
5040_wholemount_strong.wlz
(what is wlz format?)
Download all expression domains: EMAGE:5040_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: three stars
Text Annotation:
StructureLevelPatternNotes
future spinal cord
detected detected
future hindbrain
detected detected
future midbrain
detected detected
regionalExpressed dorso-anteriorly
optic vesicle
detected detected
mesonephros mesenchyme
detected detected
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:Sfrp2 probeB
Entity Detected:Sfrp2, secreted frizzled-related protein 2 ( MGI:108078)
Sequence:sense strand is shown

>Sfrp2 probeB
TTCGGCACAGGCCTCGTCACTCGCTCTCGTGCAGTGAAGGACCGCTGCGCCCGGTCATGTCCGCCTTCGC
TTCCCCTGGCCAGACATGCTGGAGTGCGACCGTTTCCCGCAGACAACGACCTCTGCATCCCCCTCGCTAG
TAGCGACCACCTCCTGCCGGCCACAGAGGAAGCTCCCAAGGTGTGTGAAGCCTGCAAAACCAAGAATGAG
GACGACAACGACATCATGGAAACCCTTTGTAAAAATGACTTCGCACTGAAAATCAAAGTGAAGGAGATAA
CGTACATCAACAGAGACAC
Notes:The Sfrp2 probe used in this study by Lescher et al. 1998 [PMID:9853965] is described as follows: "A probe corresponding to approximately 1 kb of the sFRP-2 full-length cDNA was generated from the plasmid pT7T3.fz10(1.0) (Genome System EST clone W08345) by linearisation with EcoRI and transcription with T3 RNA polymerase".
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:NMRI
Age:9.5 dpc
Theiler Stage:TS15
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Fixation:4% paraformaldehyde
Staining procedure:alkaline phosphatase + BMpurple
General Information
Authors:Lescher B; Haenig B; Kispert A, 1998 [PMID:9853965] . Indexed and spatially mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1002/(SICI)1097-0177(199812)213:4<440::AID-AJA9>3.0.CO;2-6] [ PMID:9853965] Lescher B, Haenig B, Kispert A 1998 sFRP-2 is a target of the Wnt-4 signaling pathway in the developing metanephric kidney. Dev Dyn (213):440-51
Links: Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEMAGE