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EMAGE:5041

Sh3gl2 SH3-domain GRB2-like 2 ( MGI:700009)
TS15 (9.5 dpc)
in situ hybridisation

Data Images
EMAGE:5041
Figure 4D. So CW; Sham MH; Chew SL; Cheung N; So CK; Chung SK; Caldas C; Wiedemann LM; Chan LC, "Expression and protein-binding studies of the EEN gene family, new interacting partners for dynamin, synaptojanin and huntingtin proteins." Biochem J 2000 Jun 1;348 Pt 2():447-58.

Expression pattern clarity: three stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:5041Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
5041_wholemount_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:5041_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: three stars
Text Annotation:
StructureLevelPatternNotes
embryo
not detected not detected
homogeneousNo expression could be detected in 9.5 dpc embryos by whole-mount ISH
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:Sh3gl2 probeA
Entity Detected:Sh3gl2, SH3-domain GRB2-like 2 ( MGI:700009)
Notes:The Sh3gl2 (EEN-B1) probe used in this study by So et al., 2000 [PMID:10816441] was "an 800-bp fragment spanning part of the coding sequence and 5'-untranslated region of SH3p4/endophilin I (murine EEN-B1)" and was "kindly given by B. Kay, University of Wisconsin-Madison, Madison, WI, U.S.A.".
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Age:9.5 dpc
Theiler Stage:TS15
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Fixation:4% paraformaldehyde
General Information
Authors:So CW; Sham MH; Chew SL; Cheung N; So CK; Chung SK; Caldas C; Wiedemann LM; Chan LC, 2000 [PMID:10816441] . Indexed and spatially mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1042/0264-6021:3480447] [ PMID:10816441] So CW, Sham MH, Chew SL, Cheung N, So CK, Chung SK, Caldas C, Wiedemann LM, Chan LC 2000 Expression and protein-binding studies of the EEN gene family, new interacting partners for dynamin, synaptojanin and huntingtin proteins. Biochem J (348 Pt 2):447-58
Links: Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEMAGE