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EMAGE:5045

Six3os1 Six3 opposite strand transcript 1 ( MGI:1925118)
TS17 (10.5 dpc)
in situ hybridisation

Data Images
EMAGE:5045
Figure 3B. [doi:10.1093/hmg/ddi084] Alfano G; Vitiello C; Caccioppoli C; Caramico T; Carola A; Szego MJ; McInnes RR; Auricchio A; Banfi S, "Natural antisense transcripts associated with genes involved in eye development." Hum Mol Genet 2005 Apr 1;14(7):913-23. [PMID:15703187]

Expression pattern clarity: three stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:5045Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
5045_wholemount_notDetected_3D_1.wlz
5045_wholemount_strong_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:5045_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: three stars
Text Annotation:
StructureLevelPatternNotes
optic cup
detected detected
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:Six3os1 probeA
Entity Detected:Six3os1, Six3 opposite strand transcript 1 ( MGI:1925118)
Sequence:sense strand is shown

>Six3os1 probeA
AGCTCTGAGCGCGACCCTACCCGGCTCGGGCCCGCAGCAACCCCGACTGTCCGAGAGCCTCTCCCCAAGC
AAGGCTCTGCGCCGCCCTCTGAGCCCACCTCCTGGCCACAACGCTGCCTGCGCTTCTCCAGCCCAGGCCG
GAAGCCCTATGCTGCCTCCCGAAGCCGGACTTTCTGGAAGCCCATCTTCGTATGCTCACCAGCCCACCCT
CCTACGCTGGTCAGAGTGAGATTGCTAACCTCAAGACCCCATTCAATGCCAGCTTTCAACTACTGCTGAA
GACCAATGTAGGTCCTGGAATAGAAGAACTGCAGAGTATCCCTAAGGTCTCCTCTTCATCTGGCATGTGT
GTTCACAGAAGGTACAGTGTGCGAGAACACACTTAGGAAATTTTAGGAACGCTAACCACAGTACTGGAGG
AATAAAGATGTGTCGAAGGAATGGATGGAATGCATGAACACATACACCTTGTTGTCCAGATGCAGACAAA
AGCTACCATGTCCGCTTGGAGTGACGCCCAGACACGCACAGAGATGTACAGGAAGCCAAGGAGAGTGGGA
TCCACCAGAGTACAGTACAAGTACAGAGTACAGTGCAAGATGCCCAGT
nt 53 - nt 660 of NR_015386.1
Notes:The Six3os1 probe used in this study by Alfano et al., 2005 [PMID:15703187] was generated by RT-PCR on mouse adult retina cDNA using the following primers: Six3AS-F AGCTCTGAGCGCGACCCTAC and Six3AS-R2 ACTGGGCATCTTGCACTGTACTC to make a 610bp product. Editors note: This information is taken from Supplementary Table 3 therein.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Age:10.5 dpc
Theiler Stage:TS17
Mutations:none (wild-type)
Preparation:wholemount
Procedures
General Information
Authors:Alfano G; Vitiello C; Caccioppoli C; Caramico T; Carola A; Szego MJ; McInnes RR; Auricchio A; Banfi S, 2005 [PMID:15703187] . Indexed and spatially mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1093/hmg/ddi084] [ PMID:15703187] Alfano G, Vitiello C, Caccioppoli C, Caramico T, Carola A, Szego MJ, McInnes RR, Auricchio A, Banfi S 2005 Natural antisense transcripts associated with genes involved in eye development. Hum Mol Genet (14):913-23
Links: Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEMAGE