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EMAGE:5048

Slc12a5 solute carrier family 12, member 5 ( MGI:1862037)
TS18 (11.5 dpc)
in situ hybridisation

Data Images
EMAGE:5048
Figure 1C. Copyright: This image is from [doi:10.1002/cne.10983] Stein V; Hermans-Borgmeyer I; Jentsch TJ; Hubner CA, "Expression of the KCl cotransporter KCC2 parallels neuronal maturation and the emergence of low intracellular chloride." J Comp Neurol 2004 Jan 1;468(1):57-64. Reprinted with permission of Wiley-Liss Inc. [PMID:14648690]

Expression pattern clarity: three stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
Note: mo, medulla oblongata; sc, spinal cord; t, telencephalon.
Expression Pattern Description
Spatial Annotation:
EMAGE:5048Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
5048_wholemount_moderate.wlz
5048_wholemount_possible.wlz
5048_wholemount_notDetected.wlz
5048_wholemount_strong.wlz
5048_wholemount_weak.wlz
(what is wlz format?)
Download all expression domains: EMAGE:5048_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: three stars
Text Annotation:
StructureLevelPatternNotes
future spinal cord
detected detected
Expression restricted to the spinal cord and the immature brainstem, including the developing metencephalon.
midbrain
detected detected
Expression restricted to the spinal cord and the immature brainstem, including the developing metencephalon.
telencephalon
not detected not detected
homogeneousEvolving hemispheres remained essentially unlabeled during embryonic development.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:Slc12a5 probeA
Entity Detected:Slc12a5, solute carrier family 12, member 5 ( MGI:1862037)
Sequence:sense strand is shown

>Slc12a5 probeA
CTGGCCAACTACACCAACCTACCCCAGGGAAGTAGAGAGCATGAAGAAGCAGAAAATAATGAGGGTGGAA
AAAAGAAGCCGGTGCAGGCTCCTCGAATGGGCACCTTCATGGGTGTGTACCTGCCGTGCCTGCAGAACAT
CTTTGGTGTCATCCTCTTCCTGCGGCTCACGTGGGTGGTGGGCATCGCGGGCATCATGGAGTCCTTCTGT
ATGGTCTTCATTTGCTGCTCCTGTACGATGCTCACAGCCATTTCCATGAGTGCAATCGCAACCAATGGTG
TTGTGCCTGCTGGTGGCTCGTACTACATGATTTCCAGGTCTCTGGGCCCGGAGTTTGGGGGCGCCGTGGG
CCTCTGCTTCTACCTGGGCACCACCTTTGCTGGGGCTATGTACATCCTTGGCACGATCGAGATCCTGCTG
GCTTATCTCTTCCCAGCTATGGCCATCTTCAAGGCAGAAGATGCCAGTGGGGAGGCGGCCGCCATGCTGA
ACAACATGC
nt 274 - nt 772 of NM_020333.1
Notes:The Slc12a5 (Kcc2) probe used in this study by Stein et al., 2004 [PMID:14648690] corresponds "to amino acids 63-229".
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:11.5 dpc
Theiler Stage:TS18
Mutations:none (wild-type)
Preparation:wholemount
Procedures
General Information
Authors:Stein V; Hermans-Borgmeyer I; Jentsch TJ; Hubner CA, 2004 [PMID:14648690] . Indexed and spatially mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1002/cne.10983] [ PMID:14648690] Stein V, Hermans-Borgmeyer I, Jentsch TJ, H�bner CA 2004 Expression of the KCl cotransporter KCC2 parallels neuronal maturation and the emergence of low intracellular chloride. J Comp Neurol (468):57-64
Links: Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEMAGE