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EMAGE:5054

Slit2 slit homolog 2 (Drosophila) ( MGI:1315205)
TS16 (10.5 dpc)
in situ hybridisation

Data Images
EMAGE:5054 EMAGE:5054
Figure 5E. Copyright: Reprinted with permission from Elsevier from [doi:10.1006/dbio.1999.9371] Dev Biol 212: 290-306, Yuan W; Zhou L; Chen JH; Wu JY; Rao Y; Ornitz DM, "The mouse SLIT family: secreted ligands for ROBO expressed in patterns that suggest a role in morphogenesis and axon guidance." Copyright 1999 [PMID:10433822] Figure 5F. Copyright: Reprinted with permission from Elsevier from [doi:10.1006/dbio.1999.9371] Dev Biol 212: 290-306, Yuan W; Zhou L; Chen JH; Wu JY; Rao Y; Ornitz DM, "The mouse SLIT family: secreted ligands for ROBO expressed in patterns that suggest a role in morphogenesis and axon guidance." Copyright 1999 [PMID:10433822]

Expression pattern clarity: three stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
Note: second (II) branchial arch; commissural plate (cp); eye (ey); floor plate (fp); limb bud (lb); maxillary component (ma); mandibular component (mb); nasal pit (np); rhombic lip (rl); roof plate (rp); tectum (tc).
Expression Pattern Description
Spatial Annotation:
EMAGE:5054Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
5054_wholemount_moderate_3D_1.wlz
5054_wholemount_notDetected_3D_1.wlz
5054_wholemount_strong_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:5054_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Text Annotation:
StructureLevelPatternNotes
future brain
detected detected
regionalExpressed in commissural plate, roof plate, floor plate and tectum.
neural tube floor plate
detected detected
neural tube roof plate
detected detected
future hindbrain
detected detected
regionalExpressed in rhombic lip.
eye
detected detected
olfactory pit
detected detected
otocyst
detected detected
3rd branchial groove
detected detected
1st branchial groove
detected detected
2nd branchial groove
detected detected
future forebrain
detected detected
regionalExpressed in the developing tectum.
future midbrain
detected detected
regionalExpressed in the developing tectum.
visceral organ system
detected detected
regionalExpressed in visceral grooves.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:Slit2 probeA
Entity Detected:Slit2, slit homolog 2 (Drosophila) ( MGI:1315205)
Sequence:sense strand is shown

>Slit2 probeA
GTGGACTGTCATGGGCTGGCACTGCGCAGTGTGCCCAGGAATATCCCCCGCAACACCGAGAGACTGGATT
TGAATGGAAATAACATCACGAGGATCACGAAGACAGATTTTGCTGGTCTCAGGCACCTCAGAGTTCTTCA
GCTCATGGAGAACAGAATCAGCACCATCGAGAGGGGAGCATTCCAGGATCTTAAGGAGCTGGAAAGACTG
CGTTTAAACAGAAATAACCTTCAGTTGTTTCCTGAGCTGCTGTTTCTCGGGACTGCGAAGCTCTACCGGC
TTGATCTCAGTGAAAATCAAATTCAAGCAATTCCAAGGAAGGCTTTCCGTGGGGCAGTTGACATTAAAAA
CCTGCAACTGGATTACAACCAGATCAGCTGCATTGAAGATGGGGCGTTCAGAGCTCTACGAGATCTGGAA
GTGCTCACTCTGAACAATAACAATATTACTAGACTTTCAGTGGCAAGTTTCAACCATATGCCTAAACTTA
GGACATTTCGACTCCACTCGAACAACTTGTACTGCGACTGCCACCTAGCCTGGCTCTCAGACTGGCTTCG
CCAAAGGCCACGGGTGGGCTTGTACACTCAGTGTATGGGCCCATCCCACCTGAGGGGCCACAATGTAGCA
GAGGTTCAAAAACGAGAGTTTGTCTGCAGTGGTCACCAGTCATTCATGGCTCCCTCCTGCAGTGTGCTGC
ACTGCCCCGCTGCTTGTACCTGTAGCAACAACATTGTAGACTGCCGAGGGAAAGGTCTCACTGAGATCCC
CACAAATCTGCCTGAGACCATCACAGAAATACGTTTGGAACAGAACTCCATCAGGGTCATCCCTCCAGGA
GCCTTCTCACCATACAAAAAGCTTAGACGACTAGACCTGAGCAACAACCAGATCTCTGAACTTGCACCAG
ATGCCTTCCAAGGACTGCGCTCTCTGAATTCACTTGTCCTGTATGGAAATAAAATCACAGAACTCCCAAA
AAGTTTATTCGAAGGACTATTTTCCTTGCAGCTACTATTATTGAATGCCAACAAGATAAACTGCCTTCGG
GTAGATGCTTTTCAGGACCTGCACAACTTGAACCTTCTCTCCTTATATGACAATAAGCTTCAGACGGTTG
CCAAGGGCACCTTCTCAGCCCTCAGAGCCATCCAAACTATGCATTTGGCCCAGAATCCTTTCATTTGTGA
CTGCCATCTCAAGTGGCTAGCGGATTATCTCCACACCAACCCAATTGAGACCAGCGGTGCCCGTTGCACC
AGCCCCCGCCGCCTGGCAAACAAAAGAATTGGACAGATCAAAAGCAAGAAATTCCGTTGTTCAGGTA
nt 361 - nt 1687 of AF144628.1
Notes:The Slit2 probe used in this study by Yuan et al., 1999 [PMID:10433822] was a "1327-bp PCR fragment corresponding to position 114 to 1440 bp of the coding region". Editors Note: the coding region of AF144628.1 begins at nt247 therefore 114 to 1440 bp of the coding region extends between nt 361-1687 in this sequence.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:Swiss Webster
Age:10.5 dpc
Theiler Stage:TS16
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Fixation:4% paraformaldehyde
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Yuan W; Zhou L; Chen JH; Wu JY; Rao Y; Ornitz DM, 1999 [PMID:10433822] . Indexed and spatially mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1006/dbio.1999.9371] [ PMID:10433822] Yuan W, Zhou L, Chen JH, Wu JY, Rao Y, Ornitz DM 1999 The mouse SLIT family: secreted ligands for ROBO expressed in patterns that suggest a role in morphogenesis and axon guidance. Dev Biol (212):290-306
Links: Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEMAGE