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EMAGE:506

Wnt3a wingless-related MMTV integration site 3A ( MGI:98956)
TS16 (9.5 dpc)
in situ hybridisation

Data Images
EMAGE:506
Figure 5Q of Koizumi et al., 2001 [PMID:11262239] Copyright: This image is from Development and is displayed with the permission of The Company of Biologists Ltd. who owns the copyright.

Expression pattern clarity: three stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:506Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
506_wholemount_strong_3D_1.wlz
506_wholemount_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:506_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: three stars
Text Annotation:
StructureLevelPatternNotes
tail mesenchyme
detected detected
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:1334562
Entity Detected:Wnt3a, wingless-related MMTV integration site 3A ( MGI:98956)
Sequence:sense strand is shown

>MGI:1334562
GAATTCCAATTTGGGCCGGAAGTCCTACCTCAATGGCTTGGACTCCTCTCTTGACCCGACAGGGCTCAAA
TGGAGACAGGTAAGCTACTCCCTCAACTAGGTGGGGTTCGTGCGGATGGGTGGGAGGGGAGAGATTAGGG
TCCCTCCTCCCAGAGGCACTGCTCTATCTAGATACATGAGAGGGTGCTTCAGGGTGGGCCCTATTTGGGC
TTGAGGATCCCGTGGGGGCGGGGCTTCACCCCGACTGGGTGGAACTTTTGGAGACCCCCTTCCACTGGGG
CAAGGCTTCACTGAAGACTCATGGGATGGAGCTCCACGGAAGGAGGAGTTCCTGAGCGAGCCTGGGCTCT
GAGCAGGCCATCCAGCTCCCATCTGGCCCCTTTCCAGTCCTGGTGTAAGGTTCAACCTGCAAGCCTCATC
TGCGCAGAGCAGGATCTCCTGGCAGAATGAGGCATGGAGAAGAACTCAGGGGTGATACCAAGACCTAACA
AACCCCGTGCCTGGGTACCTCTTTTAAAGCTCTGCACCCCTTCTTCAAGGGCTTTCCTAGTCTCCTTGGC
AGAGCTTTCCTGAGGAAGATTTGCAGTCCCCCAGAGTTCAAGTGAACACCCATAGAACAGAACAGACTCT
ATCCTGAGTAGAGAGGGTTCTCTAGGAATCTCTATGGGGACTGCTAGGAAGGATCCTGGGCATGACAGCC
TCGTATGATAGCCTGCATCCGCTCTGACACTTAATACTCA
nt 1452 - nt 2191 of X56842.1
Notes:The Wnt3a probe used in this study by Koizumi et al., 2001 [PMID:11262239] is indicated as that used by Takada et al., 1994 [PMID:8299937] which was previously described by Roelink & Nusse, 1991 [PMID:2001840] as a "750-bp EcoRI-BglII fragment","derived from noncoding and nonconserved cDNA fragments".
Chemistry:RNA
Strand:antisense
Specimen
Organism:mouse
Age:9.5 dpc
Theiler Stage:TS16
Mutations:none (wild-type)
Preparation:wholemount
Procedures
General Information
Authors:Koizumi et al., 2001 [PMID:11262239] Indexed by GXD, Spatially Mapped by EMAGE
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ PMID:11262239] Koizumi K, Nakajima M, Yuasa S, Saga Y, Sakai T, Kuriyama T, Shirasawa T, Koseki H 2001 The role of presenilin 1 during somite segmentation. Development (128):1391-402
 [ doi:10.1101/gad.8.2.174] [ PMID:8299937] Takada S, Stark KL, Shea MJ, Vassileva G, McMahon JA, McMahon AP 1994 Wnt-3a regulates somite and tailbud formation in the mouse embryo. Genes Dev (8):174-89
 [ doi:10.1101/gad.5.3.381] [ PMID:2001840] Roelink H, Nusse R 1991 Expression of two members of the Wnt family during mouse development--restricted temporal and spatial patterns in the developing neural tube. Genes Dev (5):381-8
Links:MGI:1932860 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI