Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:5060

Smad2 MAD homolog 2 (Drosophila) ( MGI:108051)
TS15 (10.5 dpc)
in situ hybridisation

Data Images
EMAGE:5060
Figure 1B. Baker JC; Harland RM, "A novel mesoderm inducer, Madr2, functions in the activin signal transduction pathway." Genes Dev 10: 1880-9 (1996). [PMID:8756346]

Expression pattern clarity: three stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:5060Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
5060_wholemount_moderate_3D_1.wlz
5060_wholemount_possible_3D_1.wlz
5060_wholemount_notDetected_3D_1.wlz
5060_wholemount_strong_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:5060_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
embryo
detected detected
Widely expressed, apart from in the heart and tail-bud
heart
not detected not detected
homogeneous
tail
not detected not detected
homogeneous
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:Smad2 probeA
Entity Detected:Smad2, MAD homolog 2 (Drosophila) ( MGI:108051)
Sequence:sense strand is shown

>Smad2 probeA
CAAAGTATGGACACAGGCTCTCCGGCTGAACTGTCTCCTACTACTCTCTCTCCTGTTAATCACAGCTTGG
ATTTGCAGCCAGTTACTTACTCGGAACCTGCATTCTGGTGTTCAATCGCATACTATGAACTAAACCAGAG
GGTTGGAGAGACCTTCCATGCGTCACAGCCCTCGCTCACTGTAGACGGCTTCACAGACCCATCAAACTCG
GAGAGGTTCTGCTTAGGCTTGCTCTCCAACGTTAACCGAAATGCCACTGTAGAAATGACAAGAAGACATA
TAGGAAGGGGAGTGCGCTTGTATTACATAGGTGGGGAAGTGTTTGCTGAGTGCCTAAGTGATAGTGCAAT
CTTTGTGCAGAGCCCCAACTGTAACCAGAGATACGGCTGGCACCCTGCAACAGTGTGTAAGATCCCACCA
GGCTGTAACCTGAAGATCTTCAACAACCAAGAATTTGCTGCTCTTCTGGCTCAGTCTGTCAACCAGGGTT
TTGAAGCCGTTTATCAGCTAACCCGAATGTGCACCATAAGAATGAGTTTTGTGAAGGGCTGGGGAGCAGA
ATATCGGAGGCAGACAGTAACAAGTACTCCTTGCTGGATTGAACTTCATCTGAATGGCCCTCTGCAGTGG
CTGGACAAAGTATTAACTCAGATGGGATCCCCTTCAGTGCGATGCTCAAGCATGTCGTAA
nt 774 - nt 1463 of U60530.1
Notes:The Smad2 (Madr2) probe used in this study by Baker & Harland, 1996 [PMID:8756346] was generated by RT-PCR using the following primers: "Madr2(C) U-(5'-GGAATTCATCGAGATCTCAAAGTATGGACACAGGCTCTCCGGCTGAA-3'); D-(5'-CGGAATTCTTACGACATGCTTGAGCATCGCACTGAAGG-3')".
Chemistry:RNA
Strand:antisense
Specimen
Organism:mouse
Age:10.5 dpc
Theiler Stage:TS15
Mutations:none (wild-type)
Preparation:wholemount
Procedures
General Information
Authors:Baker JC & Harland RM, 1996 [PMID:8756346] . Indexed and spatially mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1101/gad.10.15.1880] [ PMID:8756346] Baker JC, Harland RM 1996 A novel mesoderm inducer, Madr2, functions in the activin signal transduction pathway. Genes Dev (10):1880-9
Links: Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEMAGE