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EMAGE:5062

Smarcb1 SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily b, member 1 ( MGI:1328366)
TS15 (9.5 dpc)
in situ hybridisation

Data Images
EMAGE:5062
Figure 2B. [doi:10.1073/pnas.250492697] Copyright: This image is from Roberts CW; Galusha SA; McMenamin ME; Fletcher CD; Orkin SH, Proc Natl Acad Sci U S A 2000; Dec 97: 13796-800. [PMID:11095756] Copyright 2000 National Academy of Sciences, U.S.A.

Expression pattern clarity: two stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:5062Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
5062_wholemount_moderate.wlz
5062_wholemount_strong.wlz
5062_wholemount_notDetected.wlz
5062_wholemount_possible.wlz
(what is wlz format?)
Download all expression domains: EMAGE:5062_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: three stars
Text Annotation:
StructureLevelPatternNotes
branchial arch
strong strong
limb
strong strong
embryo
weak weak
homogeneousWidespead diffuse expression.
nervous system
strong strong
regionalExpressed in head folds/neuroepithelium.
heart
not detected not detected
homogeneousExpression is low to absent within the heart.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:Smarcb1 probeA
Entity Detected:Smarcb1, SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily b, member 1 ( MGI:1328366)
Sequence:sense strand is shown

>Smarcb1 probeA
GCTGGAGGACGACGGGGAGTTCTACATGATCGGCTCCGAGGTGGGAAACTACCTGCGTATGTTCCGAGGT
TCTCTGTACAAGAGATACCCCTCACTCTGGCGGCGACTAGCCACTGTGGAAGAAAGGAAGAAAATAGTGG
CATCGTCACATGGTAAAAAAACAAAACCTAACACTAAGGATCATGGATATACCACCCTGGCCACCAGCGT
GACACTCCTGAAAGCCTCAGAGGTAGAAGAGATCCTGGATGGCAATGACGAGAAGTACAAGGCTGTGTCC
ATCAGCACAGAGCCCCCGACCTACCTCAGGGAGCAGAAGGCCAAGAGGAACAGCCAGTGGGTCCCCACCC
TGCCCAACAGCTCCCACCACCTGGATGCTGTGCCCTGTTCCACCACCATCAACAGGAACCGCATGGGTCG
GGACAAGAAGAGAACCTTCCC
nt 247 - nt 687 of NM_011418.1
Notes:The Smarcb1 (Snf5) probe used in this study by Roberts et al., 2000 [PMID:11095756] a "440-bp PvuII fragment (bases 54-493 relative to the initiation codon)". Editors note: the initiation codon is at position 194 in the mouse Smarcb1 cDNA RefSeq NM_011418.1.
Chemistry:RNA
Strand:antisense
Specimen
Organism:mouse
Age:9.5 dpc
Theiler Stage:TS15
Mutations:none (wild-type)
Preparation:wholemount
Procedures
General Information
Authors:Roberts CW; Galusha SA; McMenamin ME; Fletcher CD; Orkin SH, 2000 [PMID:11095756] . Indexed and spatially mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1073/pnas.250492697] [ PMID:11095756] Roberts CW, Galusha SA, McMenamin ME, Fletcher CD, Orkin SH 2000 Haploinsufficiency of Snf5 (integrase interactor 1) predisposes to malignant rhabdoid tumors in mice. Proc Natl Acad Sci U S A (97):13796-800
Links: Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEMAGE