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EMAGE:5068

Efnb2 ephrin B2 ( MGI:105097)
TS15 (9.5 dpc)
in situ hybridisation

Data Images
EMAGE:5068
Figure 2B. Adams RH; Wilkinson GA; Weiss C; Diella F; Gale NW; Deutsch U; Risau W; Klein R. Roles of ephrinB ligands and EphB receptors in cardiovascular development: demarcation of arterial/venous domains, vascular morphogenesis, and sprouting angiogenesis. 1999 Feb 1;13(3):295-306. [PMID:9990854]

Expression pattern clarity: two stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
Note: dorsal aorta (white arrow); aortic arches (white arrowhead).
Expression Pattern Description
Spatial Annotation:
EMAGE:5068Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
5068_wholemount_moderate_3D_1.wlz
5068_wholemount_weak_3D_1.wlz
5068_wholemount_possible_3D_1.wlz
5068_wholemount_notDetected_3D_1.wlz
5068_wholemount_strong_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:5068_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: three stars
Text Annotation:
StructureLevelPatternNotes
dorsal aorta
detected detected
branchial arch artery
detected detected
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:Efnb2 probeA
Entity Detected:Efnb2, ephrin B2 ( MGI:105097)
Sequence:sense strand is shown

>Efnb2 probeA
ATGGCCCGGTCCAGGAGGGACTCTGTGTGGAAGTACTGTTGGGGACTTTTGATGGTTTTGTGCAGAACTG
CGATCTCCAGATCGATAGTTTTAGAGCCTATCTACTGGAATTCCTCGAACTCCAAATTTCTACCCGGACA
AGGCCTGGTACTATACCCACAGATAGGAGACAAATTGGATATTATTTGCCCCAAAGTGGACTCTAAAACT
GTTGGCCAGTATGAATATTATAAAGTTTATATGGTTGATAAAGACCAAGCAGACAGATGCACAATTAAGA
AGGAGAATACCCCGCTGCTCAACTGTGCCAGACCAGACCAAGATGTGAAATTCACCATCAAGTTTCAAGA
ATTCAGCCCTAACCTCTGGGGTCTAGAATTTCAGAAGAACAAAGATTACTACATTATATCTACATCAAAT
GGGTCTTTGGAGGGCCTGGATAACCAGGAGGGAGGGGTGTGCCAGACAAGAGCCATGAAGATCCTCATGA
AAGTTGGACAAGATGCAAGTTCTGCTGGATCAGCCAGGAATCACGGTCCAACAAGACGTCCAGAGCTAGA
AGCTGGTACAAATGGGAGAAGTTCAACAACAAGTCCCTTTGTGAAGCCAAATCCAGGTTCTAGCACCGAT
GGCAACAGCGCGGGGCATTCCGGGAACAATCTCCTGGGTTCCGAAGTGGCCTTATTCGCAGGGATCGCAT
CAGGATGCATCATCTTCATCGTCATCATCATCACTTTGGTGGTGCTGCTGCTCAAGTACCGCAGGAGACA
CCGCAAACACTCTCCACAGCACACGACCACGCTGTCTCTCAGCACACTGGCCACGCCCAAGCGAGGTGGC
AACAACAATGGCTCGGAGCCCAGTGACGTTATCATACCGCTAAGGACTGCA
nt 4 - nt 894 of U30244.1
Notes:The Efnb2 (ephrinB2/ELF-2) probe used in this study by Adams et al., 1999 [PMID:9990854] was a "890-bp fragment extending from nucleotide 4 to 894 (Bergemann et al. 1995 [PMID:7651410] )". Editors Note: The cDNA sequence of Efnb2 (ELF-2) shown in Fig1 of Bergemann et al. 1995 is U30244.1.
Chemistry:RNA
Strand:antisense
Specimen
Organism:mouse
Age:9.5 dpc
Theiler Stage:TS15
Mutations:none (wild-type)
Preparation:wholemount
Procedures
General Information
Authors:Adams RH; Wilkinson GA; Weiss C; Diella F; Gale NW; Deutsch U; Risau W; Klein R, 1999 [PMID:9990854] . Indexed by GXD, Spatially mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1101/gad.13.3.295] [ PMID:9990854] Adams RH, Wilkinson GA, Weiss C, Diella F, Gale NW, Deutsch U, Risau W, Klein R 1999 Roles of ephrinB ligands and EphB receptors in cardiovascular development: demarcation of arterial/venous domains, vascular morphogenesis, and sprouting angiogenesis. Genes Dev (13):295-306
 [ PMID:7651410] Bergemann AD, Cheng HJ, Brambilla R, Klein R, Flanagan JG 1995 ELF-2, a new member of the Eph ligand family, is segmentally expressed in mouse embryos in the region of the hindbrain and newly forming somites. Mol Cell Biol (15):4921-9
Links: Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEMAGE