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EMAGE:5069

Ephb3 Eph receptor B3 ( MGI:104770)
TS15 (9.5 dpc)
in situ hybridisation

Data Images
EMAGE:5069
Figure 2E. [doi:10.1101/gad.13.3.295] Genes Dev. 1999. 13:295-306. Adams RH; Wilkinson GA; Weiss C; Diella F; Gale NW; Deutsch U; Risau W; Klein R. Roles of ephrinB ligands and EphB receptors in cardiovascular development: demarcation of arterial/venous domains, vascular morphogenesis, and sprouting angiogenesis.

Expression pattern clarity: two stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:5069Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
5069_wholemount_moderate_3D_1.wlz
5069_wholemount_weak_3D_1.wlz
5069_wholemount_possible_3D_1.wlz
5069_wholemount_notDetected_3D_1.wlz
5069_wholemount_strong_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:5069_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: three stars
Text Annotation:
StructureLevelPatternNotes
branchial arch artery
detected detected
anterior cardinal vein
detected detected
posterior cardinal vein
detected detected
sinus venosus
detected detected
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:Ephb3 probeA
Entity Detected:Ephb3, Eph receptor B3 ( MGI:104770)
Sequence:sense strand is shown

>Ephb3 probeA
GGGGAATTTGGGGAAGTGTGCCGTGGTCGACTGAAACAGCCTGGCCGCCGAGAGGTGTTTGTGGCCATCA
AGACGCTGAAGGTGGGCTACACCGAGAGGCAGCGGCGGGACTTCCTAAGCGAGGCCTCCATCATGGGTCA
GTTTGATCACCCCAATATAATCCGGCTCGAGGGCGTGGTCACCAAAAGTCGGCCAGTTATGATCCTCACT
GAGTTCATGGAAAACTGCGCCCTGGACTCCTTCCTCCGGCTCAACGATGGGCAGTTCACGGTCATCCAGC
TGGTGGGCATGTTGCGGGGCATTGCTGCCGGCATGAAGTACCTGTCCGAGATGAACTATGTGCACCGCGA
CCTGGCTGCTCGCAACATCCTTGTCAACAGCAACCTGGTCTGCAAAGTCTCAGACTTTGGCCTCTCCCGC
TTCCTGGAGGATGACCCCTCCGATCCTACCTACACCAGTTCCCTGGGCGGGAAGATCCCCATCCGCTGGA
CTGCCCCAGAGGCCATAGCCTATCGGAAGTTCACTTCTGCTAGTGATGTCTGGAGCTACGGAATTGTCAT
GTGGGAGGTCATGAGCTATGGAGAGCGACCCTACTGGGACATGAGCAACCAGGATGTCATCAATGCCGTG
GAGCAGGATTACCGGCTGCCACCACCCATGGACTGTCCCACAGCACTGCACCAGCTCATGCTGGACTGCT
GGGTGCGGGACCGGAACCTCAGGCCCAAATTCTCCC
nt 1951 - nt 2686 of X75208.1
Notes:The Ephb3 (Sek4/HEK2) probe used in this study by Adams et al., 1999 [PMID:9990854] is indicated as that originally described by Orioli, 1996 [PMID:8947026] i.e. as "a 735 bp long DNA fragment of Sek4 (pos. amino acid 641-885 of HEK2; Bohme et al., 1993 [PMID:93390963] )". See Figure 2 of Bohme et al. (and the accompanying X75208.1 GenBank entry) for the sequence of human HEK2. Editors note: it is unclear from the description whether a human or mouse probe was used.
Chemistry:RNA
Strand:antisense
Specimen
Organism:mouse
Age:9.5 dpc
Theiler Stage:TS15
Mutations:none (wild-type)
Preparation:wholemount
Procedures
General Information
Authors:Adams RH; Wilkinson GA; Weiss C; Diella F; Gale NW; Deutsch U; Risau W; Klein R, 1999 [PMID:9990854] . Indexed by GXD, Spatially mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1101/gad.13.3.295] [ PMID:9990854] Adams RH, Wilkinson GA, Weiss C, Diella F, Gale NW, Deutsch U, Risau W, Klein R 1999 Roles of ephrinB ligands and EphB receptors in cardiovascular development: demarcation of arterial/venous domains, vascular morphogenesis, and sprouting angiogenesis. Genes Dev (13):295-306
 [ PMID:8947026] Orioli D, Henkemeyer M, Lemke G, Klein R, Pawson T 1996 Sek4 and Nuk receptors cooperate in guidance of commissural axons and in palate formation. EMBO J (15):6035-49
 [ PMID:8397371] Bohme B, Holtrich U, Wolf G, Luzius H, Grzeschik KH, Strebhardt K, Rubsamen-Waigmann H 1993 PCR mediated detection of a new human receptor-tyrosine-kinase, HEK 2. Oncogene (8):2857-62
Links: Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEMAGE