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EMAGE:5071

Ephb4 Eph receptor B4 ( MGI:104757)
TS15 (9.5 dpc)
in situ hybridisation

Data Images
EMAGE:5071
Figure 2F. [doi:10.1101/gad.13.3.295] Genes Dev. 1999. 13:295-306. Adams RH; Wilkinson GA; Weiss C; Diella F; Gale NW; Deutsch U; Risau W; Klein R. Roles of ephrinB ligands and EphB receptors in cardiovascular development: demarcation of arterial/venous domains, vascular morphogenesis, and sprouting angiogenesis.

Expression pattern clarity: two stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:5071Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
5071_wholemount_moderate_3D_1.wlz
5071_wholemount_weak_3D_1.wlz
5071_wholemount_possible_3D_1.wlz
5071_wholemount_notDetected_3D_1.wlz
5071_wholemount_strong_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:5071_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: three stars
Text Annotation:
StructureLevelPatternNotes
anterior cardinal vein
detected detected
posterior cardinal vein
detected detected
sinus venosus
detected detected
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:Ephb4 probeA
Entity Detected:Ephb4, Eph receptor B4 ( MGI:104757)
Sequence:sense strand is shown

>Ephb4 probeA
GAGCGCTGCTGTGCTGGGCTTCCCTCGCCACTGCTTTAGAAGAGACCCTGTTGAACACAAAACTGGAAAC
GGCGGATCTGAAATGGGTGACTTACCCTCAGGCAGAGGGCCAGTGGGAGGAGCTAAGCGGCCTGGATGAG
GAACAGCACAGCGTCCGCACCTATGAGGTGTGCGACATGAAGCGTCCAGGGGGCCAGGCTCACTGGCTGC
GCACTGGCTGGGTCCCAAGGCGAGGTGCTGTCCACGTGTATGCCACGATACGCTTCACCATGATGGAATG
CCTGTCCCTGCCGAGGGCCAGTCGCTCCTGCAAGGAGACATTCACTGTCTTCTATTACGAGAGCGAACGT
GATACGGCCACGGCCCATACGCCCGCCTGGATGGAGAACCCCTACATCAAGGTGGACACAGTGGCCGCAG
AACATCTGACTCGGAAGCGCCCTGGAGCTGAAGCCACAGGGAAAGTTAATATCAAGACGCTGCGCCTGGG
TCCTCTCAGCAAAGCTGGCTTCTACCTGGCTTTCCAGGACCAAGGAGCCTGCATGGCTCTGCTCTCCCTG
CATCTCTTTTACAAGAAGTGCTCCTGGCTGATCACGAACTTGACCTACTTCCCCGAGACGGTGCCTCGGG
AGCTCGTGGTGCCGGTGGCAGGTAGCTGCGTGGCCAACGCGGTCCCTACCGCCAACCCCAGCCCCAGCCT
CTACTGCCGGGAAGATGGTCAATGGGCTGAGCAGCAGGTCACGGGCTGCAGCTGCGCGCCAGGGTACGAG
GCTGCGGAAAGCAACAAAGTATGCAGAGCCTGTGGCCAGGGAACCTTCAAGCCCCAAATAGGAGACGAGT
CCTGCCTGCCGTGCCCAGCCAACAGCCACTCGAATAACATTGGGTCTCCTGTCTGCCTGTGTCGAATTGG
GTATTACCGGGCCCGCTCAGACCCCCGGAGTTCACCTTGCACTACCCCACCCTCTGCTCCAAGAAGCGTG
GTTCACCATTTGAATGGTTCCACCCTGCGCCTGGAATGGAGTGCTCCCCTTGAGTCCGGAGGCCGAGAGG
ACCTCACTTATGCTGTTCGCTGCCGAGAGTGCCGTCCTGGGGGTTCCTGCTTGCCCTGTGGGGGCGACAT
GACCTTCGACCCCGGTCCTCGAGACCT
nt 104 - nt 1250 of Z49085.1
Notes:The Ephb3 (MDK2) probe used in this study by Adams et al., 1999 [PMID:9990854] was "a 1146-bp fragment extending from nucleotide 104 to 1250 (Ciossek et al., 1995 [PMID:7478528] )". See Figure 1A of Ciossek et al and the accompanying Z49085.1 GenBank entry for the sequence referred to by Adams et al.
Chemistry:RNA
Strand:antisense
Specimen
Organism:mouse
Age:9.5 dpc
Theiler Stage:TS15
Mutations:none (wild-type)
Preparation:wholemount
Procedures
General Information
Authors:Adams RH; Wilkinson GA; Weiss C; Diella F; Gale NW; Deutsch U; Risau W; Klein R, 1999 [PMID:9990854] . Indexed by GXD, Spatially mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1101/gad.13.3.295] [ PMID:9990854] Adams RH, Wilkinson GA, Weiss C, Diella F, Gale NW, Deutsch U, Risau W, Klein R 1999 Roles of ephrinB ligands and EphB receptors in cardiovascular development: demarcation of arterial/venous domains, vascular morphogenesis, and sprouting angiogenesis. Genes Dev (13):295-306
 [ PMID:7478528] Ciossek T, Lerch MM, Ullrich A 1995 Cloning, characterization, and differential expression of MDK2 and MDK5, two novel receptor tyrosine kinases of the eck/eph family. Oncogene (11):2085-95
Links: Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEMAGE