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EMAGE:5081

Ebf2 early B-cell factor 2 ( MGI:894332)
TS17 (10 dpc)
in situ reporter

Data Images
EMAGE:5081
FigS1e10. Copyright: Reprinted with permission from Cell Press from [doi:10.1016/j.devcel.2005.10.009] Dev Cell 9(6): 757-67, Kieslinger M; Folberth S; Dobreva G; Dorn T; Croci L; Erben R; Consalez GG; Grosschedl R, EBF2 regulates osteoblast-dependent differentiation of osteoclasts. Copyright 2005 [PMID:16326388]

Expression pattern clarity: three stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:5081Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
5081_wholemount_moderate_3D_1.wlz
5081_wholemount_weak_3D_1.wlz
5081_wholemount_notDetected_3D_1.wlz
5081_wholemount_strong_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:5081_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: three stars
Text Annotation:
StructureLevelPatternNotes
ear
strong strong
regionalStaining around the otic vesicle can be observed, and additionally in areas of somitic differentiation.
trunk somite
strong strong
regionalStaining around the otic vesicle can be observed, and additionally in areas of somitic differentiation.
brain
detected detected
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ reporter
Entity Detected:Ebf2, early B-cell factor 2 ( MGI:894332)
Notes:Ebf2 expression was detected in this study by Kieslinger et al., 2005 [PMID:16326388] in embryos heterozygous for a targeted mutation that was generated and described by Corradi et al., 2003 [PMID:12466206] . The line was generated as such: A floxed neo cassette was removed from Ebf2.tm1Ggc via in vivo cre mediated recombination. The lacZ gene remained in frame and in place of the 5.2 kb fragment containing the coding regions of the first five exons. The deleted region included sequence encoding the majority of the DNA binding domain. The absence of full length transcript was confirmed by RT-PCR analysis of homozygous mutant mice. lacZ expression was determined to be driven by the endogenous promoter.
Labelled with:LacZ
Specimen
Organism:mouse
Strain:involves: 129S2/SvPas
Age:10 dpc
Theiler Stage:TS17
Mutations:Ebf2tm1.1Ggc/Ebf2+
Preparation:wholemount
Procedures
General Information
Authors:Kieslinger M; Folberth S; Dobreva G; Dorn T; Croci L; Erben R; Consalez GG; Grosschedl R, 2005 [PMID:16326388] , Indexed and Spatially Mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1016/j.devcel.2005.10.009] [ PMID:16326388] Kieslinger M, Folberth S, Dobreva G, Dorn T, Croci L, Erben R, Consalez GG, Grosschedl R 2005 EBF2 regulates osteoblast-dependent differentiation of osteoclasts. Dev Cell (9):757-67
 [ doi:10.1242/dev.00215] [ PMID:12466206] Corradi A, Croci L, Broccoli V, Zecchini S, Previtali S, Wurst W, Amadio S, Maggi R, Quattrini A, Consalez GG 2003 Hypogonadotropic hypogonadism and peripheral neuropathy in Ebf2-null mice. Development (130):401-10
Links:MGI:2446576 allele description
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEMAGE