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EMAGE:510

Lefty2 left-right determination factor 2 ( MGI:2443573)
TS10 (7.25 dpc)
in situ hybridisation

Data Images
EMAGE:510
Fig 6E of Perea-Gomez et al., 2001 [PMID:11171400] . Copyright: This image is from Development and is displayed with the permission of the Company of Biologists Ltd. who owns the copyright.

Expression pattern clarity: three stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
This embryo is also described by Perea-Gomez et al., 2001 [PMID:11171400] as "late streak" in the text.
Expression Pattern Description
Spatial Annotation:
EMAGE:510Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
510_wholemount_strong_3D_1.wlz
510_wholemount_moderate_3D_1.wlz
510_wholemount_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:510_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
primitive streak
detected detected
regionalExpression is detected in mesodermal cells exiting the middle part of the primitive streak.
embryo mesoderm
detected detected
regionalExpression is detected in mesodermal cells exiting the middle part of the primitive streak.
Annotation Validation: Submitter + EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:1278391
Entity Detected:Lefty2, left-right determination factor 2 ( MGI:2443573)
Notes:Leftb (Lefty-2) probe used in this study by Perea-Gomez et al., 2001 [PMID:11171400] is indicated as being originally described by Meno et al., 1997 [PMID:9348041] ie. a "BglII-EcoRI 0.5 kb fragment" from the 3'UTR. When indexed, no nucleotide sequence for the 3'UTR was available in public sequence archives to define the probe sequence here.
Chemistry:RNA
Strand:antisense
Label:undefined
Specimen
Organism:mouse
Age:7.25 dpc
Theiler Stage:TS10
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Fixation:4% paraformaldehyde
Staining procedure:undefined
General Information
Authors:Perea-Gomez et al., 2001 [PMID:11171400] Indexed by GXD, Spatially mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ PMID:11171400] Perea-Gomez A, Lawson KA, Rhinn M, Zakin L, Brulet P, Mazan S, Ang SL 2001 Otx2 is required for visceral endoderm movement and for the restriction of posterior signals in the epiblast of the mouse embryo. Development (128):753-65
 [ doi:10.1046/j.1365-2443.1997.1400338.x] [ PMID:9348041] Meno C, Ito Y, Saijoh Y, Matsuda Y, Tashiro K, Kuhara S, Hamada H 1997 Two closely-related left-right asymmetrically expressed genes, lefty-1 and lefty-2: their distinct expression domains, chromosomal linkage and direct neuralizing activity in Xenopus embryos. Genes Cells (2):513-24
Links:MGI:1930290 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI