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EMAGE:5103

Spata6 spermatogenesis associated 6 ( MGI:1915196)
TS17 (10.5 dpc)
in situ hybridisation

Data Images
EMAGE:5103
Fig 3Ba-right from: Oh C; Aho H; Shamsadin R; Nayernia K; Muller C; Sancken U; Szpirer C; Engel W; Adham IM. 2003 "Characterization, expression pattern and chromosomal localization of the spermatogenesis associated 6 gene (Spata6)". Mol Hum Reprod 9:321-30. [PMID:12771232] .

Expression pattern clarity: three stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:5103Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
5103_wholemount_moderate.wlz
5103_wholemount_weak.wlz
5103_wholemount_possible.wlz
5103_wholemount_notDetected.wlz
5103_wholemount_strong.wlz
(what is wlz format?)
Download all expression domains: EMAGE:5103_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
limb
detected detected
regionalExpressed distally.
trunk somite
detected detected
central nervous system
detected detected
regionalExpressed in dorsal neural tube
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:Spata6 probeA
Entity Detected:Spata6, spermatogenesis associated 6 ( MGI:1915196)
Sequence:sense strand is shown

>Spata6 probeA
ATTCAAAAAGGAAACAGATAAGCCTCCATTTGTGATTAGACATGTTGATCCCCCGAGTCCCAGAACTGAC
AATTTTTTTGGATCACCTGGCAGAGACTGTGAACGAGATGGATGGGTCAGGATGCACAGCGATCATCCTC
ATATTGGCTGCTGCAGATCCAAGGACTACAAGGTTATCAGGTCACCACATGGGAGAGACTTTGAGGATCC
CTTTGAAAGGTGTGAGGAATATTTGAGCCCAAGGACATGTAGCAAGCCCCAGCATTCTGCAAGGACCTTA
CTAGTCCATTCAGCACCTTCAACAACACCAAAGCATTGTGCAAGCCCTGTGCTAAATAGAGCCTCTCTCA
GGGAAAGATTCCACTCTGATTGGTGTTCACCTCCGAACTGTGATGAGATCCATGACCGGGTAAAAGATGT
CTTGAAATCACATCAAGCTCATGCAAGACATTTATGTGATGAGAGAGACCCAGAGAGAGAAGACGAACTG
GAACTGAAAAGAAGTCTTTTATATAGAGACTCTGCTTATGACAGTGACCCTGAGTATAGCTCCTTTCAGC
GGCCGCGTGGCTCTTTCCATTTGGATGATGGCGAATGCTGGTCCAACAGAGCAGCCTCTTGTAAAGGGAA
ATCCCACCGACCCGTCTTTGAGAACAGCATGGACAAGATGTACAGGAACTTATACAAAAAAGCCTGTAGT
TCTGTTTCTCATACACAGGAAAGCTTCTGAGACCATCTACCATAAACTGTATAAGCGTCCATGTCAACCT
CTCAATGAAAGCGTTTGATGTGATCTGT
nt 935 - nt 1732 of NM_026470.3
Notes:The Spata6 probe used in this study by Oh et al., 2003 [PMID:12771232] was "prepared from pBluescript KS (Stratagene) plasmid containing a rS 0.8 cDNA fragment (base pairs 1529-2329)". Editors Note: the nucleotide numbering used by the authors refers to the sequence shown in Fig4 therein. The probe region equates to nt 935-1732 of the mouse Spata6 mRNA Ref Seq NM_026470.3.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Age:10.5 dpc
Theiler Stage:TS17
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Fixation:4% paraformaldehyde
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Oh C; Aho H; Shamsadin R; Nayernia K; Muller C; Sancken U; Szpirer C; Engel W; Adham IM. 2003 [PMID:12771232] . Indexed and spatially mapped by EMAGE
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1093/molehr/gag047] [ PMID:12771232] Oh C, Aho H, Shamsadin R, Nayernia K, Muller C, Sancken U, Szpirer C, Engel W, Adham IM 2003 Characterization, expression pattern and chromosomal localization of the spermatogenesis associated 6 gene (Spata6). Mol Hum Reprod (9):321-30
Links: Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEMAGE