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EMAGE:5104

Sphk1 sphingosine kinase 1 ( MGI:1316649)
TS18 (10.5 dpc)
in situ hybridisation

Data Images
EMAGE:5104
Fig4E of [doi:10.1128/MCB.25.24.11113-11121.2005] Mol Cell Biol 2005 25(24):11113-21 [PMID:16314531] Mizugishi K; Yamashita T; Olivera A; Miller GF; Spiegel S; Proia RL, "Essential role for sphingosine kinases in neural and vascular development." © ASM. Reproduced with permission from Molecular and Cellular Biology. Reuse of any Licensed Material requires permission from ASM. Copyright 2005.

Expression pattern clarity: two stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:5104Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
5104_wholemount_moderate.wlz
5104_wholemount_weak.wlz
5104_wholemount_possible.wlz
5104_wholemount_notDetected.wlz
5104_wholemount_strong.wlz
(what is wlz format?)
Download all expression domains: EMAGE:5104_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
brain
detected detected
SphK1 was expressed in the whole brain, with the highest levels in the telencephalon
telencephalon
strong strong
SphK1 was expressed in the whole brain, with the highest levels in the telencephalon
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:Sphk1 probeA
Entity Detected:Sphk1, sphingosine kinase 1 ( MGI:1316649)
Notes:The Sphk1 probe used in this study by Mizugishi et al., 2005. [PMID:16314531] was transcribed from either a "cDNA fragment corresponding to the entire open reading frame" or "the fragment including part of the C terminus and 3' untranslated region of mouse SphK1". "The two antisense probes produced the same staining pattern. Sense probes did not show any specific signals". Editors Note: It is not specified which probe was used on this specimen.
Chemistry:RNA
Strand:antisense
Specimen
Organism:mouse
Age:10.5 dpc
Theiler Stage:TS18
Mutations:none (wild-type)
Preparation:wholemount
Procedures
General Information
Authors:Mizugishi K; Yamashita T; Olivera A; Miller GF; Spiegel S; Proia RL. 2005. [PMID:16314531] . Indexed and spatially mapped by EMAGE
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1128/MCB.25.24.11113-11121.2005] [ PMID:16314531] Mizugishi K, Yamashita T, Olivera A, Miller GF, Spiegel S, Proia RL 2005 Essential role for sphingosine kinases in neural and vascular development. Mol Cell Biol (25):11113-21
Links: Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEMAGE