EMAGE mouse gene expression spatial database

EMAGE:5121

Tagln transgelin ( MGI:106012)
TS17 (10.5 dpc)
in situ reporter

Data Images


Fig5A. Copyright: Reprinted with permission from Elsevier from [doi:10.1006/dbio.2002.0819] Dev Biol 251: 157-66, Kochilas L; Merscher-Gomez S; Lu MM; Potluri V; Liao J; Kucherlapati R; Morrow B; Epstein JA, The role of neural crest during cardiac development in a mouse model of DiGeorge syndrome. Copyright 2002 [PMID:12413905]	Fig5D. Copyright: Reprinted with permission from Elsevier from [doi:10.1006/dbio.2002.0819] Dev Biol 251: 157-66, Kochilas L; Merscher-Gomez S; Lu MM; Potluri V; Liao J; Kucherlapati R; Morrow B; Epstein JA, The role of neural crest during cardiac development in a mouse model of DiGeorge syndrome. Copyright 2002 [PMID:12413905]

Expression pattern clarity:
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Notes:

Fig5A shows a wholemount specimen and Fig5D shows a section through the branchial arches. The aortic arches are numbered.

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

wholemount mapping

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Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

3rd arch artery

detected

4th arch artery

detected

6th arch artery

detected

Annotation Validation:

EMAGE Editor

Detection Reagent

Type:	in situ reporter
Entity Detected:	Tagln, transgelin ( MGI:106012)
Notes:	Assessment of Tagln (SM22alpha) expression in this study by Kochilas et al., 2002 [PMID:12413905] "utilized a recombinant mouse in which lacZ had been inserted into the SM22 locus as a sensitive assay for early smooth muscle differentiation (Zhang et al, 2001 [PMID:11158319] )". In this targeted mutation, exon 2, intron 2, exon 3, and part of intron 3 were replaced with an in-frame lacZ gene inserted at the initiation codon followed by a neomycin selection cassette. Northern and western blot analysis demonstrated that no detectable wild type transcript or protein was expressed from this allele. Histochemical analysis demonstrated that beta-galactosidase was expressed from this allele under the control of the endogenous promoter.
Labelled with:	LacZ

Specimen

Procedures

General Information

Authors:	Kochilas L; Merscher-Gomez S; Lu MM; Potluri V; Liao J; Kucherlapati R; Morrow B; Epstein JA. 2002 [PMID:12413905] . Indexed and spatially mapped by EMAGE
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	non-screen
References:	[ doi:10.1006/dbio.2002.0819] [ PMID:12413905] Kochilas L, Merscher-Gomez S, Lu MM, Potluri V, Liao J, Kucherlapati R, Morrow B, Epstein JA 2002 The role of neural crest during cardiac development in a mouse model of DiGeorge syndrome. Dev Biol (251):157-66
	[ doi:10.1128/MCB.2001.21.4.1336-1344.2001] [ PMID:11158319] Zhang JC, Kim S, Helmke BP, Yu WW, Du KL, Lu MM, Strobeck M, Yu Q, Parmacek MS 2001 Analysis of SM22alpha-deficient mice reveals unanticipated insights into smooth muscle cell differentiation and function. Mol Cell Biol (21):1336-44
Links:	MGI:2183575 allele description
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source