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EMAGE:5122

Tagln transgelin ( MGI:106012)
TS15 (9.5 dpc)
in situ hybridisation

Data Images
EMAGE:5122
Fig6-E9.5-Tagln. [doi:10.1002/dvdy.10307] Copyright: This image is from Porter GA Jr; Makuck RF; Rivkees SA, " Intracellular calcium plays an essential role in cardiac development." Dev Dyn 2003 Jun;227(2):280-90. Reprinted with permission of Wiley-Liss Inc. [PMID:12761855] .

Expression pattern clarity: two stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
Image annotation: oft - outflow tract. Scale bar = 1mm.
Expression Pattern Description
Spatial Annotation:
EMAGE:5122Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
5122_wholemount_moderate.wlz
5122_wholemount_possible.wlz
5122_wholemount_notDetected.wlz
5122_wholemount_strong.wlz
(what is wlz format?)
Download all expression domains: EMAGE:5122_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: three stars
Text Annotation:
StructureLevelPatternNotes
common atrial chamber cardiac muscle
detected detected
Expressed in differentiated cardiac myocytes
bulbus cordis caudal half cardiac muscle
detected detected
Expressed in differentiated cardiac myocytes
bulbus cordis rostral half cardiac muscle
detected detected
Expressed in differentiated cardiac myocytes
primitive ventricle cardiac muscle
detected detected
Expressed in differentiated cardiac myocytes
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:Tagln probeA
Entity Detected:Tagln, transgelin ( MGI:106012)
Sequence:sense strand is shown

>Tagln probeA
GCTACTCTCCTTCCAGTCCACAAACGACCAAGCCTTCTCTGCCTCAACATGGCCAACAAGGGTCCATCCT
ACGGCATGAGCCGAGAAGTGCAGTCCAAAATTGAGAAGAAGTATGACGAGGAGCTGGAGGAGCGACTAGT
GGAGTGGATTGTAGTGCAGTGTGGCCCTGATGTAGGCCGCCCAGATCGTGGGCGCCTGGGCTTCCAGGTG
TGGCTGAAGAATGGTGTGATTCTGAGCAAATTGGTGAACAGCCTGTATCCTGAGGGATCGAAGCCAGTGA
AGGTGCCTGAGAACCCACCCTCCATGGTCTTTAAGCAGATGGAACAGGTGGCTCAATTCTTGAAGGCAGC
TGAAGATTATGGAGTCATCAAGACTGACATGTTCCAGACTGTTGACCTCTATGAAGGTAAGGATATGGCA
GCAGTGCAGAGGACTCTAATGGCTTTGGGCAGTTTGGCTGTGACCAAAAACGATGGAAACTACCGTGGAG
ATCCCAACTGGTTTATGAAGAAAGCCCAGGAGCATAAGAGGGACTTCACAGACAGCCAACTGCAGGAGGG
GAAGCACGTCATTGGCCTTCAAATGGGCAGCAACAGAGGAGCCTCGCAGGCTGGCATGACAGGCTATGGG
CGACCCCGGCAGATCATCAGTTAGAAAGGGAAGGCCAGCCCTGAGCTGCAGCATCCTGCTTAGCCTGCCT
CACAAATGCCTATGTAGGTTCTTAGCCCTGACAGCTCTGAGGTGTCACTGGGCAAAGATGACTGCACATG
GGCAGCTCC
nt 30 - nt 808 of NM_011526.4
Notes:The Tagln probe used in this study by Porter et al.,. 2003 [PMID:12761855] was "produced from (a) PCR product by using Topo-cloning (Invitrogen, Carlsbad, CA) and labeled by using a kit from Roche Diagnostics Corporation (Indianapolis, IN). Oligonucleotide primers (for PCR amplification) were designed against the mouse sequence of Tagln: 5'-GCTACTCTCCTTCCAGTCCAC-3' , 5'-GGAGCTGCCCATGTGCAGTC-3'."
Chemistry:RNA
Strand:antisense
Specimen
Organism:mouse
Strain:C57BL/6J
Age:9.5 dpc
Theiler Stage:TS15
Mutations:none (wild-type)
Preparation:wholemount
Procedures
General Information
Authors:Porter GA Jr; Makuck RF; Rivkees SA. 2003 [PMID:12761855] . Indexed and spatially mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1002/dvdy.10307] [ PMID:12761855] Porter GA, Makuck RF, Rivkees SA 2003 Intracellular calcium plays an essential role in cardiac development. Dev Dyn (227):280-90
Links: Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEMAGE