Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:5124

Klf2 Kruppel-like factor 2 (lung) ( MGI:1342772)
TS15 (9.5 dpc)
in situ hybridisation

Data Images
EMAGE:5124
Fig5B (erk5+/-, E9.5) of [doi:10.1128/MCB.25.19.8553-8566.2005] Mol Cell Biol 2005 25(19):8553-8566 [PMID:16166637] Sohn SJ; Li D; Lee LK; Winoto A, "Transcriptional Regulation of Tissue-Specific Genes by the ERK5 Mitogen-Activated Protein Kinase." © ASM. Reproduced with permission from Molecular and Cellular Biology. Reuse of any Licensed Material requires permission from ASM. Copyright 2005.

Expression pattern clarity: two stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
Image annotations: endocardium (arrow); intersomitic vessels (arrowheads).
Expression Pattern Description
Spatial Annotation:
EMAGE:5124Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
5124_wholemount_moderate_3D_1.wlz
5124_wholemount_weak_3D_1.wlz
5124_wholemount_possible_3D_1.wlz
5124_wholemount_notDetected_3D_1.wlz
5124_wholemount_strong_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:5124_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
outflow tract endocardial tube
detected detected
Expression associated with endocardium.
cardiovascular system
detected detected
regionalExpression associated with intersomitic vessels
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:Klf2 cloneA
Entity Detected:Klf2, Kruppel-like factor 2 (lung) ( MGI:1342772)
Notes:The Klf2 (LKLF) probe template used in this study by Sohn et al., 2005 [PMID:16166637] is described as a "cDNA clone (a 500-bp LKLF cDNA fragment amplified by PCR)" from a microarray library discussed in the paper.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Age:9.5 dpc
Theiler Stage:TS15
Mutations:Mapk7tm1Wnt/Mapk7+
Preparation:wholemount
Procedures
Fixation:4% paraformaldehyde
Staining procedure:alkaline phosphatase + BMpurple
General Information
Authors:Sohn SJ; Li D; Lee LK; Winoto A, 2005 [PMID:16166637] . Indexed and spatially mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1128/MCB.25.19.8553-8566.2005] [ PMID:16166637] Sohn SJ, Li D, Lee LK, Winoto A 2005 Transcriptional regulation of tissue-specific genes by the ERK5 mitogen-activated protein kinase. Mol Cell Biol (25):8553-66
Links:MGI:2445409 allele description
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEMAGE