EMAGE mouse gene expression spatial database

EMAGE:5126

Arf6 ADP-ribosylation factor 6 ( MGI:99435)
TS15 (10.5 dpc)
in situ hybridisation

Data Images


Fig2K(left) of [doi:10.1128/MCB.00298-06] Mol Cell Biol 2006 26(16):6149-6156 [PMID:16880525] Suzuki T; Kanai Y; Hara T; Sasaki J; Sasaki T; Kohara M; Maehama T; Taya C; Shitara H; Yonekawa H; Frohman MA; Yokozeki T; Kanaho Y, "Crucial role of the small GTPase ARF6 in hepatic cord formation during liver development." © ASM. Reproduced with permission from Molecular and Cellular Biology. Reuse of any Licensed Material requires permission from ASM. Copyright 2006.

Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Notes:

Image annotations: The arrowhead indicates the liver, which stained intensely with the probe.

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

wholemount mapping

Download individual expression domains:

Download all expression domains:

Find spatially similar wholemount expression patterns:

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

septum transversum hepatic component

strong

regional

The liver is stained intensely with the probe.

Annotation Validation:

EMAGE Editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	Arf6 probeA
Entity Detected:	Arf6, ADP-ribosylation factor 6 ( MGI:99435)
Sequence:	sense strand is shown >Arf6 probeA GATCTTCGGGAACAAGGAAATGCGGATCCTCATGCTGGGCCTGGACGCAGCCGGCAAGACAACGATCCTG TACAAGTTGAAGCTGGGCCAATCGGTGACCACCATCCCCACGGTGGGCTTCAACGTGGAGACGGTGACTT ACAAAAACGTCAAGTTCAACGTGTGGGATGTGGGCGGCCAGGACAAGATCCGGCCGCTCTGGCGGCATTA CTACACCGGGACCCAGGGTCTGATCTTCGTGGTAGACTGCGCCGACCGCGACCGCATCGACGAGGCCCGC CAGGAGCTGCACCGCATTATCAATGACCGGGAGATGAGGGACGCCATCATCCTCATCTTCGCCAACAAGC AGGACCTGCCCGATGCCATGAAACCCCATGAGATCCAGGAGAAACTGGGCCTGACCCGGATTCGGGACAG GAACTGGTATGTGCAGCCCTCCTGTGCCACCTCCGGGGACGGACTCTATGAGGGGCTCACATGGTT
	nt 507 - nt 992 of NM_007481.1
Notes:	The Arf6 probe used in this study by Suzuki et al., 2006 [PMID:16880525] is described as follows: "To synthesize a probe to detect ARF6 mRNA expression, an ARF6 cDNA, a generous gift of K. Nakayama, was digested with HincII, subcloned into pBluescript II SK(+), and then digested with BglII. Using the digested plasmid, a digoxigenin-labeled antisense probe corresponding to nucleotides 507 to 992 of the ARF6 gene was generated using a DIG RNA-labeling kit (Roche)." Editors note: Digesting the Arf6 cDNA RefSeq NM_007481 (version 1) with BglII and HincII is consistent with the nucleotide numbering given by the authors.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Procedures

General Information

Authors:	Suzuki T; Kanai Y; Hara T; Sasaki J; Sasaki T; Kohara M; Maehama T; Taya C; Shitara H; Yonekawa H; Frohman MA; Yokozeki T; Kanaho Y, 2006 [PMID:16880525] , Indexed and Spatially Mapped by EMAGE.
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	non-screen
References:	[ doi:10.1128/MCB.00298-06] [ PMID:16880525] Suzuki T, Kanai Y, Hara T, Sasaki J, Sasaki T, Kohara M, Maehama T, Taya C, Shitara H, Yonekawa H, Frohman MA, Yokozeki T, Kanaho Y 2006 Crucial role of the small GTPase ARF6 in hepatic cord formation during liver development. Mol Cell Biol (26):6149-56
Links:	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source