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EMAGE:5133

Capn6 calpain 6 ( MGI:1100850)
TS17 (10.5 dpc)
in situ hybridisation

Data Images
EMAGE:5133 EMAGE:5133
Fig1A of [doi:10.1128/MCB.00992-06] Mol Cell Biol 2007 27(7): 2548-2561 [PMID:17210638] Tonami K; Kurihara Y; Aburatani H; Uchijima Y; Asano T; Kurihara H, "Calpain 6 Is Involved in Microtubule Stabilization and Cytoskeletal Organization." © ASM. Reproduced with permission from Molecular and Cellular Biology. Reuse of any Licensed Material requires permission from ASM. Copyright 2007. Fig1C of [doi:10.1128/MCB.00992-06] Mol Cell Biol 2007 27(7): 2548-2561 [PMID:17210638] Tonami K; Kurihara Y; Aburatani H; Uchijima Y; Asano T; Kurihara H, "Calpain 6 Is Involved in Microtubule Stabilization and Cytoskeletal Organization." © ASM. Reproduced with permission from Molecular and Cellular Biology. Reuse of any Licensed Material requires permission from ASM. Copyright 2007.

Expression pattern clarity: three stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
Image annotations: ep, epithelium; fl, forelimb bud; hl, hindlimb bud; ht, heart; md, mandibular arch; me, mesenchyme; nt, neural tube; sm, somites. Fig1A shows the whole embryo, fig1C shows the excised mandibular arches.
Expression Pattern Description
Spatial Annotation:
EMAGE:5133Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
5133_wholemount_moderate.wlz
5133_wholemount_weak.wlz
5133_wholemount_possible.wlz
5133_wholemount_notDetected.wlz
5133_wholemount_strong.wlz
(what is wlz format?)
Download all expression domains: EMAGE:5133_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
1st branchial arch mandibular component
detected detected
limb
detected detected
trunk somite
detected detected
heart
detected detected
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:3711700
Entity Detected:Capn6, calpain 6 ( MGI:1100850)
Notes:The Capn6 probe template used in this study by Tonami et al., 2007 [PMID:17210638] is described as such: "The 3' untranslated region of Capn6 was amplified by reverse transcription (RT)-PCR of cDNA samples from E10.5 mandibular arches and subcloned into the pCRII TOPO vector (Invitrogen) for the generation of antisense riboprobes".
Chemistry:DNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Age:10.5 dpc
Theiler Stage:TS17
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Fixation:4% paraformaldehyde
General Information
Authors:Tonami K; Kurihara Y; Aburatani H; Uchijima Y; Asano T; Kurihara H, 2007 [PMID:17210638] , Indexed by GXD, Spatially Mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1128/MCB.00992-06] [ PMID:17210638] Tonami K, Kurihara Y, Aburatani H, Uchijima Y, Asano T, Kurihara H 2007 Calpain 6 is involved in microtubule stabilization and cytoskeletal organization. Mol Cell Biol (27):2548-61
Links:MGI:3711706 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI