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EMAGE:5135

Igdcc3 immunoglobulin superfamily, DCC subclass, member 3 ( MGI:1202390)
TS17 (10.5 dpc)
in situ reporter

Data Images
EMAGE:5135
Fig4B of [doi:10.1128/MCB.21.17.6031-6043.2001] Mol Cell Biol 2001 21(17): 6031-6043 [PMID:11486040] Yang W; Li C; Mansour SL, "Impaired Motor Coordination in Mice That Lack punc." © ASM. Reproduced with permission from Molecular and Cellular Biology. Reuse of any Licensed Material requires permission from ASM. Copyright 2001.

Expression pattern clarity: two stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
Image annotations: forebrain (fb), midbrain (mb), hindbrain (hb), spinal cord (sc), forelimb (fl), hindlimb (hl), branchial arch (ba), LN indicates LacZneo.
Expression Pattern Description
Spatial Annotation:
EMAGE:5135Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
5135_wholemount_moderate_3D_1.wlz
5135_wholemount_possible_3D_1.wlz
5135_wholemount_notDetected_3D_1.wlz
5135_wholemount_strong_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:5135_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
forelimb bud
strong strong
regionalevidence for restriction to the anterior
hindlimb bud
strong strong
regionalevidence for restriction to the anterior
forebrain
strong strong
regionalexpressed ventrally
hindbrain
strong strong
regionalexpressed ventrally
midbrain
strong strong
regionalexpressed ventrally
future spinal cord
strong strong
regionalexpressed ventrally
branchial arch
detected detected
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ reporter
Entity Detected:Igdcc3, immunoglobulin superfamily, DCC subclass, member 3 ( MGI:1202390)
Notes:Punc expression in this study by Yang et al., 2001. [PMID:11486040] was detected in mice heterozygous for a targeted allele whereby the gene was disrupted by insertion of a PGK-neo-lacZ cassette into exon 2 via homologous recombination, which introduces stop codons into all 3 reading frames. LoxP sites flank the PGK-neo portion of the targeting vector. Northern blot analysis of E11.5 homozygous mutant embryos did not detect gene transcripts. RT-PCR detected very low amounts of mutant transcripts thought to originate within the lacZ gene.
Labelled with:LacZ
Specimen
Organism:mouse
Strain:nvolves: 129S1/Sv * 129X1/SvJ * C57BL/6
Age:10.5 dpc
Theiler Stage:TS17
Mutations:Punctm1Sms/Punc+
Preparation:wholemount
Procedures
General Information
Authors:Yang W; Li C; Mansour SL, 2001. [PMID:11486040] . Indexed and spatially mapped by EMAGE
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1128/MCB.21.17.6031-6043.2001] [ PMID:11486040] Yang W, Li C, Mansour SL 2001 Impaired motor coordination in mice that lack punc. Mol Cell Biol (21):6031-43
Links:MGI:2180750 allele description
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEMAGE