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EMAGE:5136

Igdcc3 immunoglobulin superfamily, DCC subclass, member 3 ( MGI:1202390)
TS17 (10.5 dpc)
in situ hybridisation

Data Images
EMAGE:5136
Fig4C of [doi:10.1128/MCB.21.17.6031-6043.2001] Mol Cell Biol 2001 21(17): 6031-6043 [PMID:11486040] Yang W; Li C; Mansour SL, "Impaired Motor Coordination in Mice That Lack punc." © ASM. Reproduced with permission from Molecular and Cellular Biology. Reuse of any Licensed Material requires permission from ASM. Copyright 2001.

Expression pattern clarity: two stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
Image annotations: forebrain (fb), midbrain (mb), hindbrain (hb), spinal cord (sc), forelimb (fl), hindlimb (hl), branchial arch (ba).
Expression Pattern Description
Spatial Annotation:
EMAGE:5136Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
5136_wholemount_moderate_3D_1.wlz
5136_wholemount_weak_3D_1.wlz
5136_wholemount_possible_3D_1.wlz
5136_wholemount_notDetected_3D_1.wlz
5136_wholemount_strong_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:5136_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
branchial arch
detected detected
forelimb bud
detected detected
regionalrestricted to the anterior limb
hindlimb bud
detected detected
regionalrestricted to the anterior limb
forebrain
detected detected
regionalexpressed ventrally
hindbrain
detected detected
regionalexpressed ventrally
midbrain
detected detected
regionalexpressed ventrally
future spinal cord
detected detected
regionalexpressed ventrally
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:Punc probeA
Entity Detected:Igdcc3, immunoglobulin superfamily, DCC subclass, member 3 ( MGI:1202390)
Sequence:sense strand is shown

>Punc probeA
ACTATGAATGTGTGGCTCAGAACCGCTTTGGGCTGCTGGTCAGTCGGAAGGCTCGTCTCCAGGCTGCAAC
TATGTCCGACTTCCACGTACACCCCCAGGCCGTCACGGGTGAGGAGGGTGGTGTGGCCCGCTTCCAGTGC
CAAATCCATGGGCTTCCCAAGCCCCTGATCACATGGGAGAAGAACAGAGTTCCCATTGACACGGACGATG
AGAGGTACACACTACTGCCCAAGGGAGTCCTGCAGATAACAGGACTTCGGGCTGAGGACAGCGGCATCTT
CCACTGTGTGGCCTCAAACATTGCTAGTGTGCGGGTCAGCCACGGGGCCAGGCTCACTGTGTCAGGCTCG
GGCTCAGGGACCTACAAGGAGCCCACCATTCTTGTGGGGCCTGAGAACCTCACCCTGACAGTGCACCAGA
CTGCGGTGCTGGAGTGTGTCGCCACGGGCAACCCGCGCCCCATTGTGTCCTGGAGCCGCTTGGACGGCCG
CCCCATTGGGGTGGAGGGCATCCAGGTGCTGGGCACAGGAAACCTTATCATCTCAGATGTGACCGTCCAG
CATTCAGGTGTCTATGTCTGTGCAGCCAACAGGCCTGGCAC
nt 603 - nt 1203 of AF026465.1
Notes:The Punc probe used in this study by Yang et al., 2001 [PMID:11486040] is described as follows: "A 540-bp fragment of punc cDNA (nucleotides 603 to 1203 of GenBank accession no. AF026465) was cloned into pBluescript vectors KS(-) and SK(-). Sense and antisense digoxigenin-labeled riboprobes were synthesized using T7 RNA polymerase from the respective vectors."
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Age:10.5 dpc
Theiler Stage:TS17
Mutations:none (wild-type)
Preparation:wholemount
Procedures
General Information
Authors:Yang W; Li C; Mansour SL, 2001. [PMID:11486040] . Indexed and spatially mapped by EMAGE
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1128/MCB.21.17.6031-6043.2001] [ PMID:11486040] Yang W, Li C, Mansour SL 2001 Impaired motor coordination in mice that lack punc. Mol Cell Biol (21):6031-43
Links: Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEMAGE