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EMAGE:5141

Ctbp1 C-terminal binding protein 1 ( MGI:1201685)
TS18 (10.5 dpc)
in situ hybridisation

Data Images
EMAGE:5141 EMAGE:5141
Fig6A of Mol Cell Biol 1999 19(12): 8581-8590 [PMID:10567582] Furusawa T; Moribe H; Kondoh H; Higashi Y, "Identification of CtBP1 and CtBP2 as Corepressors of Zinc Finger-Homeodomain Factor delta EF1" © ASM. Reproduced with permission from Molecular and Cellular Biology. Reuse of any Licensed Material requires permission from ASM. Copyright 1999. Fig6C of Mol Cell Biol 1999 19(12): 8581-8590 [PMID:10567582] Furusawa T; Moribe H; Kondoh H; Higashi Y, "Identification of CtBP1 and CtBP2 as Corepressors of Zinc Finger-Homeodomain Factor delta EF1" © ASM. Reproduced with permission from Molecular and Cellular Biology. Reuse of any Licensed Material requires permission from ASM. Copyright 1999.

Expression pattern clarity: two stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
Image annotations: SC, spinal cord; H, heart. Fig6a is a lateral view of the embryo, fig6c is a dorsal view. The inset shows an embryo hybridized with the corresponding sense probe.
Expression Pattern Description
Spatial Annotation:
EMAGE:5141Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
5141_wholemount_moderate_3D_1.wlz
5141_wholemount_weak_3D_1.wlz
5141_wholemount_notDetected_3D_1.wlz
5141_wholemount_strong_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:5141_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: three stars
Text Annotation:
StructureLevelPatternNotes
future spinal cord
detected detected
heart
not detected not detected
homogeneous
embryo
detected detected
Broad expression among various tissues.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:3623780
Entity Detected:Ctbp1, C-terminal binding protein 1 ( MGI:1201685)
Sequence:sense strand is shown

>MGI:3623780
GCTGCTGTGGAAGGGATTGTTCCCAGTGCCATGTCCCTGTCTCATGGCCTGCCCCCTGTGGCCCACCCAC
CCCACGCTCCCTCTCCTGGCCAGACTGTCAAGCCTGAGGCGGATAGAGACCATACGAGTGACCAGTTGTA
GCCTGTGAGGAGCTGTCCAGCCTTGGCACCTGGTGGAGGGTGCTGCACGCTCTTGGACCCAAGTGTGCAG
AGGTGGCATCCAGTGTGGGCCCTAGCACTCCAGAGACTGGCCCGGGCGTGCACTAGGCGGGGCAAGAGGA
AGCACGTCCCCAAGTTGATTGTAGTTGTCCCGTCCTGTGGGCTGGCTCACTGTCCTGTGTCCTTTGCGTT
CCTCGTTTAAGCAAAAGAAGTTAGTAGCTAATTCTATCACGAATGTTCTTGTCTGTGTACAGTTTTTAGA
ACATTACAAAGGATTTGTTTGCTTAGCTGTCAACAAAAAGAAAACCTGAGGGAGCATTCAGCAAGTCAGT
TTGAGATTATTTTTCCTTTTCTACGTTGGAACGTGCCCCGGAATGTGGCCGTCGGCACACTTCTCAGGAC
AATGAATCCTTCCTGTTTTTCTTTTTATGCCACACAGTGCATTGTTTTTCTACCTGCTTGTCTTATTTTT
AAATAATTTAGAAAAACAACAAAGGCTGTTTTTCCTAATTTTGGCATGAACCCCTTGTTCCAAGTGAAGG
CGGCATCACGAGTGGCTCCGAGGCGTCTGCA
nt 1337 - nt 2067 of AB033122.1
Notes:The Ctbp1 probe used in this study by Furusawa et al., 1999 [PMID:10567582] is described as "The PvuII-PstI fragment (1186 to 1916) of CtBP1 cDNA." Editors note: The CtBP1 cDNA sequence deposited in GenBank from Furusawa is AB033122.1. When AB033122.1 is digested with PvuII and PstI, the resulting 730 bp fragment lies between nucleotides 1337 and 2067 (not 1186 and 1916 as indicated by the authors).
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Age:10.5 dpc
Theiler Stage:TS18
Mutations:none (wild-type)
Preparation:wholemount
Procedures
General Information
Authors:Furusawa T; Moribe H; Kondoh H; Higashi Y, 1999 [PMID:10567582] , Indexed by GXD, Spatially Mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ PMID:10567582] Furusawa T, Moribe H, Kondoh H, Higashi Y 1999 Identification of CtBP1 and CtBP2 as corepressors of zinc finger-homeodomain factor deltaEF1. Mol Cell Biol (19):8581-90
Links:MGI:3623788 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI