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EMAGE:5142

Ctbp2 C-terminal binding protein 2 ( MGI:1201686)
TS17 (10.5 dpc)
in situ hybridisation

Data Images
EMAGE:5142 EMAGE:5142
Fig6B of Mol Cell Biol 1999 19(12): 8581-8590 [PMID:10567582] Furusawa T; Moribe H; Kondoh H; Higashi Y, "Identification of CtBP1 and CtBP2 as Corepressors of Zinc Finger-Homeodomain Factor delta EF1" © ASM. Reproduced with permission from Molecular and Cellular Biology. Reuse of any Licensed Material requires permission from ASM. Copyright 1999. Fig6D of Mol Cell Biol 1999 19(12): 8581-8590 [PMID:10567582] Furusawa T; Moribe H; Kondoh H; Higashi Y, "Identification of CtBP1 and CtBP2 as Corepressors of Zinc Finger-Homeodomain Factor delta EF1" © ASM. Reproduced with permission from Molecular and Cellular Biology. Reuse of any Licensed Material requires permission from ASM. Copyright 1999.

Expression pattern clarity: two stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
Image annotations: SC, spinal cord; H, heart; CG, cephalic ganglia; DRG, dorsal root ganglia; PL, posterior-distal portion of the limb bud mesenchyme. Fig6b is a lateral view of the embryo, fig6d is a dorsal view. The inset shows an embryo hybridized with the corresponding sense probe.
Expression Pattern Description
Spatial Annotation:
EMAGE:5142Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
5142_wholemount_moderate_3D_1.wlz
5142_wholemount_weak_3D_1.wlz
5142_wholemount_notDetected_3D_1.wlz
5142_wholemount_strong_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:5142_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: three stars
Text Annotation:
StructureLevelPatternNotes
future spinal cord
detected detected
cranial ganglion
detected detected
dorsal root ganglion
detected detected
forelimb bud mesenchyme
detected detected
regionalExpression is in the posterior-distal portion.
hindlimb bud mesenchyme
detected detected
regionalExpression is in the posterior-distal portion.
heart
not detected not detected
homogeneous
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:3623781
Entity Detected:Ctbp2, C-terminal binding protein 2 ( MGI:1201686)
Sequence:sense strand is shown

>MGI:3623781
TCGGGATCGCTGTGTGCAACATCCCATCTGCTGCAGTGGAGGAGACAGCAGATTCGACAGTCTGCCACAT
TCTCAATCTGTATCGGCGGAACACATGGCTCTACCAGGCCCTCCGAGAAGGCACTCGAGTACAGAGTGTG
GAACAGATCCGTGAGGTCGCCTCAGGAGCTGCTCGGATCCGAGGGGAAACACTGGGCCTCATTGGCTTTG
GTCGCACAGGGCAGGCAGTTGCTGTTCGAGCCAAGGCCTTTGGATTCAGCGTCATATTTTATGACCCCTA
CTTACAGGATGGGATAGAACGATCTCTGGGCGTGCAAAGGGTCTACACCCTGCAGGACTTGCTATATCAG
AGCGACTGCGTCTCCTTGCACTGCAACCTGAACGAGCACAACCATCACCTCATCAATGACTTCACTATCA
AGCAGATGAGGCAAGGTGCATTCCTTGTGAATGCAGCCCGAGGTGGCCTGGTGGATGAGAAAGCCTTAGC
TCAGGCCCTCAAGGAGGGCAGGATACGAGGAGCTGCCCTGGATGTGCACGAGTCTGAGCCCTTCAGCTTC
GCCCAGGGCCCATTGAAGGATGCTCCAAATCTCATCTGCACACCACACACAGCCTGGTACAGTGAACAAG
CATCACTAGAGATGAGGGAAGCAGCAGCCACTGAGATCCGCCGAGCAATCACAGGTCGCATCCCAGAAAG
CTTACGAAACTGTGTCAACAAAGAATTCTTCGTGACTTCAGCTCCTTGGTCAGTCATTGACCAGCAAGCA
ATTCACCCCGAGCT
nt 770 - nt 1553 of AB033123.1
Notes:The Ctbp2 probe used in this study by Furusawa et al., 1999 [PMID:10567582] is described as "The SacI fragment (356-1139) of CtBP2 cDNA." Editors note: The CtBP2 cDNA sequence deposited in GenBank from Furusawa is AB033123.1. When AB033123.1 is digested with with SacI, the resulting 783 bp fragment lies between nucleotides 770 and 1553 (not 356 and 1139 as indicated by the authors).
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Age:10.5 dpc
Theiler Stage:TS17
Mutations:none (wild-type)
Preparation:wholemount
Procedures
General Information
Authors:Furusawa T; Moribe H; Kondoh H; Higashi Y, 1999 [PMID:10567582] , Indexed by GXD, Spatially Mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ PMID:10567582] Furusawa T, Moribe H, Kondoh H, Higashi Y 1999 Identification of CtBP1 and CtBP2 as corepressors of zinc finger-homeodomain factor deltaEF1. Mol Cell Biol (19):8581-90
Links:MGI:3623790 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI