Type: | in situ hybridisation probe |
Identifier: | Tcfap2e probeA |
Entity Detected: | Tfap2e, transcription factor AP-2, epsilon ( MGI:2679630) |
Sequence: | sense strand is shown
>Tcfap2e probeA
GCCTCGGAGAAGGATACCAAGCATCGGAAGTAACTGGCTCTTGTTCCCTAGCTAAATGACTCCCAGAGCC
TGAGATGGGGCCTTGGCTCCTGGGGGTAAAATGTGGGATTCTATTCAGGCCAGAGAGAACATTTTTCCAG
AAGCCCAGAGTGGGACATAGTTGGGTAAAAGAAGAAGGCTTTTCTGTGGTTTCTGGTAAGGACTCGTAAC
GCGAACTGGCATGGGAAAGTCTGGCTCAGGGGACAGAGCCCTTCCCACTGAGATGCCTGGGCAGCCATGG
GTACCCCGAGGCTGATAACACATCAGAGGTGCTATTGAAGAATTAGTGACCTTCACTTCAGTCTCTTAGT
CTCAGAACCCAGAAGGAGGGTCCAGTGGGCTCTTTGGATCCTTCAGGCTCTTGGAGACGAGAAGCTGGAG
AGACCCCTGAAGAACGAGATGGTACAGCACAAACTACTGAACTTGAACCTAGGCTGCATTACCACAGTCC
TGTTGCTGCCTGTCTTATTTATTTATATACGATCTAA
|
| nt 1575 - nt 2101 of NM_198960.2 |
Notes: | The Tcfap2e (AP-2epsilon) probe used in this study by Wang et al., 2004 [PMID:15305293] was "a 526-bp fragment obtained from the 3'UTR region of the murine AP-2epsilon cDNA (that) was excised with StuI/SalI from the murine IMAGE clone (IMAGE 778986) and subcloned into pBluescript. Digoxigenin-labeled cRNA sense and antisense probes were in vitro transcribed from the linearized plasmid".
Editors note: in GenBank, only 5' sequence is available for IMAGE:778986 (AA414551.1). In the derivation of the Knowles Solter mouse 2 cell cDNA library (which IMAGE:778986 is from), the polyT primer had a SalI site attached. There is only one StuI site in the Tcfap2e mouse cDNA RefSeq NM_198960.2 (at position 1575). A 526bp fragment extends from this site to position 2101 in NM_198960.2, which is presumed to correspond to the 3' end of the insert of IMAGE:778986. |
Chemistry: | RNA |
Strand: | antisense |
Label: | digoxigenin |