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EMAGE:5161

Tek endothelial-specific receptor tyrosine kinase ( MGI:98664)
TS15 (20 Somite no.)
immunohistochemistry

Data Images
EMAGE:5161
Fig1Ba of [doi:10.1016/S1074-7613(00)80665-2] Takakura N; Huang XL; Naruse T; Hamaguchi I; Dumont DJ; Yancopoulos GD; Suda T/ 1998 "Critical role of the TIE2 endothelial cell receptor in the development of definitive hematopoiesis." Immunity 9(5):677-86 [PMID:9846489] .

Expression pattern clarity: two stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:5161Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
5161_wholemount_moderate.wlz
5161_wholemount_possible.wlz
5161_wholemount_notDetected.wlz
5161_wholemount_strong.wlz
(what is wlz format?)
Download all expression domains: EMAGE:5161_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: three stars
Text Annotation:
StructureLevelPatternNotes
vitelline artery
detected detected
regionalTIE2+ endothelial cells and TIE2+ hematopoietic cell-aggregates in the omphalomesenteric artery (OA) and vitelline artery (VA) were found.
arterial system
detected detected
regionalTIE2+ endothelial cells and TIE2+ hematopoietic cell-aggregates in the omphalomesenteric artery (OA) and vitelline artery (VA) were found.
cardiovascular system
detected detected
regionalTIE2 expression was detected in round hematopoietic cells as well as bipolar endothelial cells
mesenchyme derived from splanchnopleure
detected detected
TIE2+ cells are detected in the P-sp (para-aortic splanchnopleural mesoderm) region.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:antibody
Identifier:Tek antibodyA
Entity Detected:Tek, endothelial-specific receptor tyrosine kinase ( MGI:98664)
Notes:The anti-TEK (TIE2) antibody used in this study by Takakura et al., 1998 [PMID:9846489] is described as "an anti-TIE2 antibody (TEK4, rat anti-mouse monoclonal) (Yano et al, 1997 [PMID:9192754] )". Editors Note: Yano et al, 1997 do not refer to any of their antisera as "TEK4" however they do refer to an "anti-TEK MoAb, clone 4", which was produced as follows: "The entire extracellular domain of murine TEK tagged with a FLAG octapeptide (DYKDDDDK) was produced by COS7 cells. Proteins in the conditioned media of COS7 cells were purified using an antiFLAG MoAb M2 affinity gel (Eastman Kodak, New Haven, CT). The Wistar rats were immunized with these purified proteins. Hybridomas were produced by fusion of murine myeloma cells (P3 - X63 - Ag8 - U1; P3U1) with splenic cells from immunized rats. Positive hybridoma clones were screened by fluorescence-activated cell sorter (FACS) analysis using BaF/TEK cells as indicators. The MoAbs were purified using a protein G-Sepharose column (Pharmacia, Uppsala, Sweden) and biotinylated."
Antibody Type:monoclonal
Raised In:rat
Specimen
Organism:mouse
Strain:C57BL/6
Age:20 Somite no.
Theiler Stage:TS15
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Secondary Antibody:anti-rat Ig antibody
Labelled with:horse radish peroxidase
Visualisation method:DAB
General Information
Authors:Takakura N; Huang XL; Naruse T; Hamaguchi I; Dumont DJ; Yancopoulos GD; Suda T. 1998 [PMID:9846489] . Indexed and spatially mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1016/S1074-7613(00)80665-2] [ PMID:9846489] Takakura N, Huang XL, Naruse T, Hamaguchi I, Dumont DJ, Yancopoulos GD, Suda T 1998 Critical role of the TIE2 endothelial cell receptor in the development of definitive hematopoiesis. Immunity (9):677-86
 [ PMID:9192754] Yano M, Iwama A, Nishio H, Suda J, Takada G, Suda T 1997 Expression and function of murine receptor tyrosine kinases, TIE and TEK, in hematopoietic stem cells. Blood (89):4317-26
Links: Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEMAGE