Type: | in situ hybridisation probe |
Identifier: | MGI:228812 |
Entity Detected: | Tfg, Trk-fused gene ( MGI:1338041) |
Sequence: | sense strand is shown
>MGI:228812
GGTGTTCAGTACTCAGCTAGTTACAGCCAGCAGACTGGACCCCAACAACCTCAGCAGTTCCAGGGATATG
GCCAACAACCAACTTCCCAGGCACCGGCTCCTGCCTTTTCTGGCCAGCCACAACAACTGCCTGCTCAACC
ACCACAGCAGTACCAGGCGAGCACTTACCCTCCACAAACGTATACTACCCAACCATCTCAGCCTGCCAAC
TATACTGTTCCCCCTGGCTCTCAGCCTGGAATGGCTCCAAGCCAACCTGGTGCTTACCAACCAAGACGCA
GGTTTTACGCCATCTCCTGCAAGTACCATGACCCCTCCTTCTAGTGGGGCTAACCCTTATGCTCGTAACC
GTCCTCCTTTTGGTCAGGGGTATGCCCAACCTGGACCTGGTTATCGATAAGGAGGTTC
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Notes: | The Tfg probe used in this study by Mencinger & Aman 1999 [PMID:10092511] is described as follows: "Antisense and sense mouse TFG RNA probes were synthesized by in vitro transcription reaction. The mouse TFG clone 367380 was linearized with EcoRI or NotI and used as a template for the generation of antisense and sense RNA probes, respectively. Probes were labeled using RNA DIG labeling kit (Roche, 1175025) according to the recommendations of the manufacturer. The T3 and T7 RNA polymerase were used for the generation of the antisense or sense probe, respectively". |
Chemistry: | RNA |
Strand: | antisense |
Label: | digoxigenin |