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EMAGE:5166

Tfg Trk-fused gene ( MGI:1338041)
TS18 (10.5 dpc)
in situ hybridisation

Data Images
EMAGE:5166 EMAGE:5166
Fig4b-right. [doi:10.1006/bbrc.1999.0417] Reprinted from Biochem Biophys Res Comm, vol 257, Mencinger M; Aman P, “Characterization of TFG in mus musculus and Caenorhabditis elegans.”, pp 67-73, Copyright (1999) with permission from Elsevier. [PMID:10092511] Fig4b-left. [doi:10.1006/bbrc.1999.0417] Reprinted from Biochem Biophys Res Comm, vol 257, Mencinger M; Aman P, “Characterization of TFG in mus musculus and Caenorhabditis elegans.”, pp 67-73, Copyright (1999) with permission from Elsevier. [PMID:10092511]

Expression pattern clarity: two stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
The authors state in this figure legend that these two embryos are stained with the antisense (Fig4b-left) and sense (Fig4b-right) mouse TFG probes. Editors Note: These left/right descriptions are presumed to have been confused as elsewhere the authors state: "A distinct staining among organs was observed by using the TFG antisense probe for ISH on 10,5 d whole embryos. The limb buds, branchial arches, nasal processes and brain stained moderately, whereas the primitive spinal cord and the dorsal root ganglia stained less. Other organs appeared negative. The sense TFG probe, used as a negative control, did not produce any signal ".
Expression Pattern Description
Spatial Annotation:
EMAGE:5166Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
5166_wholemount_moderate.wlz
5166_wholemount_weak.wlz
5166_wholemount_possible.wlz
5166_wholemount_notDetected.wlz
5166_wholemount_strong.wlz
(what is wlz format?)
Download all expression domains: EMAGE:5166_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
branchial arch
moderate moderate
limb
moderate moderate
brain
moderate moderate
nasal process
moderate moderate
future spinal cord
weak weak
dorsal root ganglion
weak weak
visceral organ system
not detected not detected
homogeneousThe authors state that expression is absent in other organs.
ear
not detected not detected
homogeneous
eye
not detected not detected
homogeneous
cardiovascular system
not detected not detected
homogeneousThe authors state that expression is absent in other organs.
skeleton
not detected not detected
homogeneousThe authors state that expression is absent in other organs.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:228812
Entity Detected:Tfg, Trk-fused gene ( MGI:1338041)
Sequence:sense strand is shown

>MGI:228812
GGTGTTCAGTACTCAGCTAGTTACAGCCAGCAGACTGGACCCCAACAACCTCAGCAGTTCCAGGGATATG
GCCAACAACCAACTTCCCAGGCACCGGCTCCTGCCTTTTCTGGCCAGCCACAACAACTGCCTGCTCAACC
ACCACAGCAGTACCAGGCGAGCACTTACCCTCCACAAACGTATACTACCCAACCATCTCAGCCTGCCAAC
TATACTGTTCCCCCTGGCTCTCAGCCTGGAATGGCTCCAAGCCAACCTGGTGCTTACCAACCAAGACGCA
GGTTTTACGCCATCTCCTGCAAGTACCATGACCCCTCCTTCTAGTGGGGCTAACCCTTATGCTCGTAACC
GTCCTCCTTTTGGTCAGGGGTATGCCCAACCTGGACCTGGTTATCGATAAGGAGGTTC
Notes:The Tfg probe used in this study by Mencinger & Aman 1999 [PMID:10092511] is described as follows: "Antisense and sense mouse TFG RNA probes were synthesized by in vitro transcription reaction. The mouse TFG clone 367380 was linearized with EcoRI or NotI and used as a template for the generation of antisense and sense RNA probes, respectively. Probes were labeled using RNA DIG labeling kit (Roche, 1175025) according to the recommendations of the manufacturer. The T3 and T7 RNA polymerase were used for the generation of the antisense or sense probe, respectively".
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Age:10.5 dpc
Theiler Stage:TS18
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Staining procedure:alkaline phosphatase + Purple alkaline phosphatase-substrate ((Roche, 1442074))
General Information
Authors:Mencinger M; Aman P. 1999 [PMID:10092511] Indexed by GXD, Spatially mapped by EMAGE
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1006/bbrc.1999.0417] [ PMID:10092511] Mencinger M, Aman P 1999 Characterization of TFG in mus musculus and Caenorhabditis elegans. Biochem Biophys Res Commun (257):67-73
Links:MGI:3604362 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI